Agrochemical control of gene expression using evolved split RNA polymerase. II.
| Autor: | Yuan Y; Department of Neurophysiology and Neuropharmacology, Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China., Miao J; Duke Kunshan University, Kunshan, Jiangsu Province, China. |
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| Jazyk: | angličtina |
| Zdroj: | PeerJ [PeerJ] 2024 Sep 04; Vol. 12, pp. e18042. Date of Electronic Publication: 2024 Sep 04 (Print Publication: 2024). |
| DOI: | 10.7717/peerj.18042 |
| Abstrakt: | Agrochemical inducible gene expression system provides cost-effective and orthogonal control of energy and information flow in bacterial cells. However, the previous version of Mandipropamid inducible gene expression system (Mandi-T7) became constitutively active at room temperature. We moved the split site of the eRNAP from position LYS179 to position ILE109. This new eRNAP showed proximity dependence at 23 °C, but not at 37 °C. We built Mandi-T7-v2 system based on the new eRNAP and it worked in both Escherichia coli and Agrobacterium tumefaciens . We also induced GFP expression in Agrobacterium cells in a semi- in vivo system. The modified eRNAP when combined with the leucine zipper-based dimerization system, behaved as a cold inducible gene expression system. Our new system provides a means to broaden the application of agrochemicals for both research and agricultural application. Portions of this text were previously published as part of a preprint (https://www.biorxiv.org/content/10.1101/2024.04.02.587689v1). Competing Interests: The authors have filed a patent application for the technology described in this article (application number: 2024102651123). (©2024 Yuan and Miao.) |
| Databáze: | MEDLINE |
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