Escherichia coli in urban marine sediments: interpreting virulence, biofilm formation, halotolerance, and antibiotic resistance to infer contamination or naturalization.

Autor:	Erb IK; Applied Microbiology, Department of Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.; Sweden Water Research AB, Ideon Science Park, Scheelevägen 15, SE-223 70 Lund, Sweden., Suarez C; Water Resources Engineering, Department of Building and Environmental Technology, Lund University, PO Box 118, SE-221 00 Lund, Sweden., Frank EM; Sweden Water Research AB, Ideon Science Park, Scheelevägen 15, SE-223 70 Lund, Sweden.; Water Resources Engineering, Department of Building and Environmental Technology, Lund University, PO Box 118, SE-221 00 Lund, Sweden., Bengtsson-Palme J; Division for Systems and Synthetic Biology, Department of Life Sciences, SciLifeLab, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden.; Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10, SE-413 46 Gothenburg, Sweden.; Centre for Antibiotic Resistance research (CARe), SE-413 45 Gothenburg, Sweden., Lindberg E; City of Helsingborg, Department of City Planning, Järnvägsgatan 22, SE-252 25 Helsingborg, Sweden., Paul CJ; Applied Microbiology, Department of Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.; Water Resources Engineering, Department of Building and Environmental Technology, Lund University, PO Box 118, SE-221 00 Lund, Sweden.
Jazyk:	angličtina
Zdroj:	FEMS microbes [FEMS Microbes] 2024 Aug 14; Vol. 5, pp. xtae024. Date of Electronic Publication: 2024 Aug 14 (Print Publication: 2024).
DOI:	10.1093/femsmc/xtae024
Abstrakt:	Marine sediments have been suggested as a reservoir for pathogenic bacteria, including Escherichia coli . The origins, and properties promoting survival of E. coli in marine sediments (including osmotolerance, biofilm formation capacity, and antibiotic resistance), have not been well-characterized. Phenotypes and genotypes of 37 E. coli isolates from coastal marine sediments were characterized. The isolates were diverse: 30 sequence types were identified that have been previously documented in humans, livestock, and other animals. Virulence genes were found in all isolates, with more virulence genes found in isolates sampled from sediment closer to the effluent discharge point of a wastewater treatment plant. Antibiotic resistance was demonstrated phenotypically for one isolate, which also carried tetracycline resistance genes on a plasmid. Biofilm formation capacity varied for the different isolates, with most biofilm formed by phylogroup B1 isolates. All isolates were halotolerant, growing at 3.5% NaCl. This suggests that the properties of some isolates may facilitate survival in marine environments and can explain in part how marine sediments can be a reservoir for pathogenic E. coli . As disturbance of sediment could resuspend bacteria, this should be considered as a potential contributor to compromised bathing water quality at nearby beaches. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© The Author(s) 2024. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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