A comprehensive apoptotic assessment of niloticin in cervical cancer cells: a tirucallane-type triterpenoid from Aphanamixis polystachya (Wall.) Parker.

Autor: Joseph AG; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in.; Academy of Scientific and Innovative Research (AcSIR) Ghaziabad 201002 India., Biji M; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in.; Academy of Scientific and Innovative Research (AcSIR) Ghaziabad 201002 India., Murali VP; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in., Sherin DR; School of Digital Sciences, Kerala University of Digital Sciences, Innovation and Technology Thiruvananthapuram-695317 India., Valsan A; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in.; Academy of Scientific and Innovative Research (AcSIR) Ghaziabad 201002 India., Sukumaran VP; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in.; Academy of Scientific and Innovative Research (AcSIR) Ghaziabad 201002 India., Radhakrishnan KV; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in., Maiti KK; CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Chemical Sciences and Technology Division (CSTD), Organic Chemistry Section Industrial Estate Thiruvananthapuram 695019 India radhu2005@gmail.com kkmaiti@niist.res.in.
Jazyk: angličtina
Zdroj: RSC medicinal chemistry [RSC Med Chem] 2024 Aug 08. Date of Electronic Publication: 2024 Aug 08.
DOI: 10.1039/d4md00318g
Abstrakt: Pharmacologically active small organic molecules derived from natural resources are prominent drug candidates due to their inherent structural diversity. Herein, we explored one such bioactive molecule, niloticin, which is a tirucallane-type triterpenoid isolated from the stem barks of Aphanamixis polystachya (Wall.) Parker. After initial screening with other isolated compounds from the same plant, niloticin demonstrated selective cytotoxicity against cervical cancer cells (HeLa) with an IC 50 value of 11.64 μM. Whereas the compound exhibited minimal cytotoxicity in normal epithelial cell line MCF-10A, with an IC 50 value of 83.31 μM. Subsequently, in silico molecular docking studies of niloticin based on key apoptotic proteins such as p53, Fas, FasL, and TNF β revealed striking binding affinity, reflecting docking scores of -7.2, -7.1, -6.8, and -7.2. Thus, the binding stability was evaluated through molecular dynamic simulation. In a downstream process, the apoptotic capability of niloticin was effectively validated through in vitro fluorimetric assays, encompassing nuclear fragmentation. Additionally, an insightful approach involving surface-enhanced Raman spectroscopy (SERS) re-establishes the occurrence of DNA cleavage during cellular apoptosis. Furthermore, niloticin was observed to induce apoptosis through both intrinsic and extrinsic pathways. This was evidenced by the upregulation of upstream regulatory molecules such as CD40 and TNF, which facilitate the activation of caspase 8. Concurrently, niloticin-induced p53 activation augmented the expression of proapoptotic proteins Bax and Bcl-2 and downregulation of IAPs, leading to the release of cytochrome C and subsequent activation of caspase 9. Therefore, the reflection of mitochondrial-mediated apoptosis is in good agreement with molecular docking studies. Furthermore, the anti-metastatic potential was evidenced by wound area closure and Ki67 expression patterns. This pivotal in vitro assessment confirms the possibility of niloticin being a potent anti-cancer drug candidate, and to the best of our knowledge, this is the first comprehensive anticancer assessment of niloticin in HeLa cells.
Competing Interests: The authors declare no competing financial interest.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
Externí odkaz: