Magnetic silica-coated cutinase immobilized via ELPs biomimetic mineralization for efficient nano-PET degradation.

Autor: Liu G; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China., Yuan H; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China., Chen Y; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China; School of Chemistry and Molecular Biology, University of Wollongong, Wollongong, NSW 2522, Australia., Mao L; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China., Yang C; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China., Zhang R; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China., Zhang G; Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China. Electronic address: zhgyghh@hqu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 279 (Pt 4), pp. 135414. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1016/j.ijbiomac.2024.135414
Abstrakt: The proliferation of nano-plastic particles (NPs) poses severe environmental hazards, urgently requiring effective biodegradation methods. Herein, a novel method was developed for degrading nano-PET (polyethylene terephthalate) using immobilized cutinases. Nano-PET particles were prepared using a straightforward method, and biocompatible elastin-like polypeptide-magnetic nanoparticles (ELPs-MNPs) were obtained as magnetic cores via biomimetic mineralization. Using one-pot synthesis with the cost-effective precursor tetraethoxysilane (TEOS), silica-coated magnetically immobilized ELPs-tagged cutinase (ET-C@SiO 2 @MNPs) were produced. ET-C@SiO 2 @MNPs showed rapid magnetic separation within 30 s, simplifying recovery and reuse. ET-C@SiO 2 @MNPs retained 86 % of their initial activity after 11 cycles and exhibited superior hydrolytic capabilities for nano-PET, producing 0.515 mM TPA after 2 h of hydrolysis, which was 96.6 % that of free enzymes. Leveraging ELPs biomimetic mineralization, this approach offers a sustainable and eco-friendly solution for PET-nanoplastic degradation, highlighting the potential of ET-C@SiO 2 @MNPs in effective nanoplastic waste management and contributing to environmental protection and sustainable development.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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