Excipient-free nanotransformation of hydrophilic macromolecules using aqueous counter collision for enhanced bioavailability.

Autor: Kim H; AMOREPACIFIC Research and Innovation Center, 1920 Yonggu-daero, Yongin-si, Gyeonggi-do 17074, Republic of Korea., Song C; AMOREPACIFIC Research and Innovation Center, 1920 Yonggu-daero, Yongin-si, Gyeonggi-do 17074, Republic of Korea., Min D; AMOREPACIFIC Research and Innovation Center, 1920 Yonggu-daero, Yongin-si, Gyeonggi-do 17074, Republic of Korea., Yoo J; AMOREPACIFIC Research and Innovation Center, 1920 Yonggu-daero, Yongin-si, Gyeonggi-do 17074, Republic of Korea., Choi J; AMOREPACIFIC Research and Innovation Center, 1920 Yonggu-daero, Yongin-si, Gyeonggi-do 17074, Republic of Korea. Electronic address: cjhstory@amorepacific.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 279 (Pt 4), pp. 135416. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1016/j.ijbiomac.2024.135416
Abstrakt: The demand for sustainable, eco-friendly biopolymer transdermal delivery systems has increased owing to growing environmental awareness. In this study, we used aqueous counter collision (ACC), a nontoxic nanotransformation method, to convert high- and ultrahigh-molecular-weight hydrophilic macromolecules into their corresponding nanoparticles (NPs). Hyaluronic acid (HA) and crosslinked HA (CLHA) were chosen as the model compounds. Their NPs exhibited particle sizes in the range of 10-100 nm and negative zeta potentials (-20 to -30 mV). Transmission electron microscopy revealed that the NPs were nearly spherical with smooth surfaces. Fourier-transform infrared and proton nuclear magnetic resonance spectroscopy and agarose gel electrophoresis confirmed that the structures and molecular weights of HA and CLHA remained unaltered after ACC. However, the storage and loss moduli of HANPs and CLHANPs were significantly lower than those of HA and CLHA, respectively. Furthermore, the permeation of HANPs and CLHANPs in reconstructed human skin and human cadaver skin was visualized and quantified. HANPs and CLHANPs penetrated deeper into the skin, whereas HA and CLHA were mainly found in the stratum corneum. The total skin absorption (permeation and deposition) of HANPs and CLHANPs was approximately 2.952 and 5.572 times those of HA and CLHA, respectively. Furthermore, HANPs and CLHANPs exhibited resistance to enzyme and free radical degradation. Our findings reveal ACC as a promising, sustainable hydrophilic macromolecule delivery system compared with the chemical hydrolysis of HA.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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