Evidence of Browning and Inflammation Features in Visceral Adipose Tissue of Women with Endometriosis.

Autor: Abobeleira JP; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Neto AC; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Mauersberger J; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Salazar M; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Botelho M; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Fernandes AS; Department of Obstetrics and Gynecology, Centro Hospitalar Universitário S. João, Porto, Portugal., Martinho M; Department of Obstetrics and Gynecology, Centro Hospitalar Universitário S. João, Porto, Portugal., Serrão MP; Department of Biomedicine-Pharmacology and Therapeutics Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; MedInUP, Center for Drug Discovery and Innovative Medicines, Porto, Portugal., Rodrigues AR; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal; Faculty of Nutrition and Food Sciences, University of Porto, Porto, Portugal., Almeida H; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Gouveia AM; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal., Neves D; Department of Biomedicine - Experimental Biology Unit, Faculty of Medicine of the University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, i3S, Porto, Portugal. Electronic address: delmagal@med.up.pt.
Jazyk: angličtina
Zdroj: Archives of medical research [Arch Med Res] 2024 Nov; Vol. 55 (7), pp. 103064. Date of Electronic Publication: 2024 Sep 07.
DOI: 10.1016/j.arcmed.2024.103064
Abstrakt: Background: Patients with endometriosis tend to have a low body mass index, suggesting an inverse relationship between body fat and risk of disease. This is supported by evidence that miRNAs differentially expressed in endometriosis induce browning of pre-adipocytes in vitro. Thus, we hypothesize that endometriosis may underlie adipose tissue (AT) dysfunction and browning.
Aims: Identify inflammation and browning processes in AT collected from endometriosis patients.
Methods: Visceral and subcutaneous AT samples were obtained during endometriosis (n = 32) or uterine myoma (n = 14; controls) surgery. Blood catecholamines were determined by high-performance liquid chromatography while IL-6 and TGF-β levels were quantified by ELISA. Adipocyte cross-sectional areas were analyzed in H&E-stained sections by computer-assisted morphometry. Macrophages (F4/80; Galectin-3) and browning activation (UCP-1; PGC-1α) in tissues were identified by dual label immunofluorescence. Expression of inflammatory (IL-6; MCP-1; Galectin-3; CD206; TIMP1; TGF-β) and browning-related (UCP-1; PGC-1α; DIO2; CITED1; CIDEA; TMEM26; TBX1; PRDM16; PPAR-γ) molecules in AT were assessed by RT-PCR and Western blotting.
Results: Compared to controls, patients presented smaller adipocytes, especially in VAT, and lower norepinephrine levels. Serum IL-6, but not TGF-β, was increased in patients. UCP-1, PGC-1α, IL-6, and MCP-1 were upregulated in VAT from endometriosis women, which also evidenced a reduction of CD206, relative to controls. However, no differences were found in mRNA expression of IL-6, TIMP1, and TGF-β nor Galectin-3 protein levels. In SAT, protein expression remained unchanged between patients and controls.
Conclusions: Our findings support an endometriosis' role as a pro-catabolic state along with local signals of VAT browning and inflammation.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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