Cu/Gd co-doped hydroxyapatite/poly lactic-co-glycolic acid composites enhance MRI imaging and bone defect regeneration.

Autor:	Lu W; Department of orthopedics, Digital orthopedic laboratory, The Second Hospital of Dalian Medical University, Dalian, P. R. China., Xia X; Department of orthopedics, Digital orthopedic laboratory, The Second Hospital of Dalian Medical University, Dalian, P. R. China., Ma Y; Department of Spine Surgery, China-Japan Union Hospital of Jilin University, Changchun, China., He H; Department of orthopedics, Digital orthopedic laboratory, The Second Hospital of Dalian Medical University, Dalian, P. R. China., Kikkawa DO; Shiley Eye Institute, San Diego, CA, USA., Zhang L; Department of orthopedics, Digital orthopedic laboratory, The Second Hospital of Dalian Medical University, Dalian, P. R. China., Zhang B; Department of Neurosurgery, The Shenzhen Luohu Hospital Group, The Third Affiliated Hospital of Shenzhen University, Shenzhen, China., Liu X; Department of orthopedics, Digital orthopedic laboratory, The Second Hospital of Dalian Medical University, Dalian, P. R. China.
Jazyk:	angličtina
Zdroj:	Journal of biomaterials applications [J Biomater Appl] 2024 Sep 08, pp. 8853282241276064. Date of Electronic Publication: 2024 Sep 08.
DOI:	10.1177/08853282241276064
Abstrakt:	Background: The hydroxyapatite (HA)/poly(lactide-co-glycolide) acid (PLGA) composite material is a widely used orthopedic implant due to its excellent biocompatibility and plasticity. Recent advancements in cation doping have expanded its potential biological applications. However, conventional HA/PLGA composites are not visible under X-rays post-implantation and have limited osteogenic induction capabilities. Copper (Cu) is known to regulate osteoblast proliferation and differentiation, while gadolinium (Gd) can significantly enhance the magnetic resonance imaging (MRI) capabilities of materials. Methods: This study aimed to investigate whether incorporating Cu and Gd into an HA/PLGA composite could enhance the osteogenic properties, in vivo bone defect repair, and MRI characteristics. We prepared a Cu/Gd@HA/PLGA composite and assessed its performance. Results: Material characterization confirmed that Cu/Gd@HA retained the morphology and crystal structure of HA. The Cu/Gd@HA/PLGA composite exhibited excellent nuclear magnetic imaging capabilities, porosity, and hydrophilicity, which are conducive to cell adhesion and implant detection. In vitro experiments demonstrated that the Cu/Gd@HA/PLGA composite enhanced the proliferation, differentiation, and adhesion of MC3T3-E1 cells, and upregulated COL-1 and BMP-2 expression at both gene and protein levels. In vivo studies showed that the Cu/Gd@HA/PLGA composite maintained strong T1-weighted MRI signals and significantly improved the bone defect healing rate in rats. Conclusion: These findings indicate that the Cu/Gd@HA/PLGA composites significantly enhance T1-weighted MRI capabilities, promote osteoblast proliferation and differentiation in vitro, and accelerate bone defect healing in vivo. Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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