Genome-wide identification of cannabinoid biosynthesis genes in non-drug type Cannabis (Cannabis sativa L.) cultivar.

Autor: Sng BJR; Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.; Department of Biological Sciences, National University of Singapore, Singapore, 117543, Singapore., Jeong YJ; Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Korea., Leong SH; Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore., Jeong JC; Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Korea., Lee J; Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Korea., Rajani S; Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore., Kim CY; Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Korea. kimcy@kribb.re.kr., Jang IC; Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore. jangi@tll.org.sg.; Department of Biological Sciences, National University of Singapore, Singapore, 117543, Singapore. jangi@tll.org.sg.
Jazyk: angličtina
Zdroj: Journal of cannabis research [J Cannabis Res] 2024 Sep 07; Vol. 6 (1), pp. 35. Date of Electronic Publication: 2024 Sep 07.
DOI: 10.1186/s42238-024-00246-8
Abstrakt: Background: Cannabis sativa cultivars can be classified as marijuana or hemp, depending on its amount of the psychoactive cannabinoid Δ 9 -tetrahydrocannabinolic acid (THCA). Hemp Cheungsam is a non-drug type Cannabis sativa that is characterized by low THCA content. However, the transcripts and expression profile of cannabinoid biosynthesis pathway genes of hemp Cheungsam have not been investigated.
Methods: RNA-sequencing (RNA-seq) was performed on three different tissue types (flower, leaf, and stem) of hemp Cheungsam to understand their transcriptomes. The expression of cannabinoid biosynthesis pathway genes was further analyzed in each tissue type. Multiple sequence alignment and conserved domain analyses were used to investigate the homologs of cannbinoid biosynthesis genes.
Results: We found that the cannabinoid biosynthesis pathway was mainly expressed in the flowers of hemp Cheungsam, similar to other Cannabis cultivars. However, expression of cannabidiolic acid (CBDA) synthase was much higher than THCA synthase and cannabichromenic acid (CBCA) synthase, suggesting that the transcription profile favors CBDA biosynthesis. Sequence analysis of cannabinoid biosynthesis pathway genes suggested the identity of orthologs in hemp Cheungsam.
Conclusions: Cannabinoid biosynthesis in hemp Cheungsam mostly occurs in the flowers, compared to other plant organs. While CBDA synthase expression is high, THCA and CBCA synthase expression is considerably low, indicating lesser THCA biosynthesis and thus low THCA content. Sequence analysis of key genes (CBDA, THCA, and CBCA synthases) of the cannabinoid biosynthetic pathway indicates that orthologs are present in hemp Cheungsam.
(© 2024. The Author(s).)
Databáze: MEDLINE
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