Comparative assessment of antibacterial activity of Matricaria chamomilla L. extract, nisin and of its combination against Alicyclobacillus spp.

Autor: de Oliveira PRS; Post-Graduate Program in Food Science, State University of Maringá, Av. Colombo, 5790, Maringá, 87020-900, Paraná, Brazil. Electronic address: prsanchesoliveira@gmail.com., Pretes NS; Post-Graduate Program in Food Science, State University of Maringá, Av. Colombo, 5790, Maringá, 87020-900, Paraná, Brazil. Electronic address: nataliapretes@gmail.com., Ribeiro AC; State University of Maringá, Department of Biotechnology, Genetics and Cell Biology, Maringá, Paraná, Brazil. Electronic address: annacarlaribeiro1@gmail.com., Castro JC; Department of Basic Health Sciences, State University of Maringá, Av. Colombo, 5790, Maringá, 87020-900, Parana, Brazil. Electronic address: jccastro2@uem.br., Garcia FP; Laboratory of Technological Innovation in the Development of Pharmaceuticals and Cosmetics, State University of Maringá, Maringá, CEP 87020-900, PR, Brazil. Electronic address: fpgarcia2@uem.br., Nakamura CV; Laboratory of Technological Innovation in the Development of Pharmaceuticals and Cosmetics, State University of Maringá, Maringá, CEP 87020-900, PR, Brazil. Electronic address: cvnakamura@uem.br., Bona E; Post-Graduate Program in Food Technology (PPGTA), Federal Technological University of Paraná (UTFPR), Campo Mourão, Paraná, Brazil; Post-Graduate Program in chemistry (PPGQ), Federal Technological University of Paraná (UTFPR), Curitiba, Paraná, Brazil. Electronic address: ebona@utfpr.edu.br., Mikcha JMG; Department of Clinical Analysis and Biomedicine, State University of Maringá, Av. Colombo, 5790, Maringá, 87020-900, Paraná, Brazil. Electronic address: jmgmikcha@uem.br., Junior MM; Department of Basic Health Sciences, State University of Maringá, Av. Colombo, 5790, Maringá, 87020-900, Parana, Brazil. Electronic address: mmjunior@uem.br., de Abreu Filho BA; Post-Graduate Program in Food Science, State University of Maringá, Av. Colombo, 5790, Maringá, 87020-900, Paraná, Brazil. Electronic address: baafilho@uem.br.
Jazyk: angličtina
Zdroj: Food microbiology [Food Microbiol] 2024 Dec; Vol. 124, pp. 104597. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1016/j.fm.2024.104597
Abstrakt: Alicyclobacillus spp. is a potential spoiling agent of acidic products and citrus drinks, leading to sensory alterations in contaminated products and consequent economic losses. Treatments such as pasteurization eliminate vegetative cells, but also create a favorable atmosphere for spore germination. To guarantee quality and safety, the application of natural substances as bioconservatives is a considerable and promising alternative for the food industry. This study evaluated the effect of hexane extract of Matricaria chamomilla L. (HE), Nisin (N) and their combination (HE + N). These compounds are present in some studies describing their antibacterial action, but no studies were found on the association of these compounds against the species Alicyclobacillus spp. This study aimed to analyze the antioxidant activity (AA) for the DPPH • (0,23 μmol Trolox/mg) and ABTS (27.93 μmol Trolox/mg), the Checkboard test revealed synergism between HE and N with a fractional inhibitory index (FIC) of 0.068., and to study the antibacterial and sporicidal effect. The antibacterial and sporicidal activity was satisfactory against Alicyclobacillus acidoterrestris with MIC and MBC of 1.95 μg/mL and MSC of 7.81 μg/mL in analyzes using HE + N. The application in orange juice proved to be effective, with an MBC of 0.007 μg/mL. The MIC results served as a parameter for other tests carried out in this study, such as flow cytometry and Scanning Electron Microscopy (SEM), and for the evaluation of sensory characteristics with Electronic Nose (E-nose).
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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