Banisteriopsis caapi extract: Implications for neuroinflammatory pathways in Locus coeruleus lesion rodent model.

Autor: Graça SC; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil. Electronic address: santhiagocalvelo@hotmail.com., Bustelli IB; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil., Santos ÉVD; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil. Electronic address: schilman.victoria@gmail.com., Fernandes CG; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil. Electronic address: carolina.fernandes@fcmsantacasasp.edu.br., Lanaro R; Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13083-894, Campinas, SP, Brazil. Electronic address: rlanaro@unicamp.br., Stilhano RS; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil. Electronic address: roberta.yamaguchi@fcmsantacasasp.edu.br., Linardi A; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil. Electronic address: alelinardi40@gmail.com., Caetano AL; Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), 01221-020, São Paulo, SP, Brazil. Electronic address: ariadiny.caetano@fcmsantacasasp.edu.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 Sep 06; Vol. 337 (Pt 1), pp. 118775. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1016/j.jep.2024.118775
Abstrakt: Ethnopharmacology Relevance: Ayahuasca is a beverage obtained from the decoctions of Banisteriopsis caapi (Spruce ex Griseb.) Morton and Psychotria viridis Ruiz & Pav., used throughout the Amazon as a medicinal beverage for healing and spiritual exploration. The Banisteriopsis caapi extract consists of harmine, harmaline, and tetrahydroharmine (THH); which inhibit the isoforms of monoamine oxidase A and B. In the central nervous system (CNS), it can increase the norepinephrine (NE) concentration, produced in the Locus coeruleus (LC), reducing inflammation that is associated with some neurological disease, such as Parkinson's disease and Alzheimer's disease.
Aim of the Study: evaluate the effects of treatment with B. caapi extract on the neuroinflammatory profile in animals with selective LC lesions.
Material and Methods: male Wistar rats with LC lesions induced by 6-hydroxydopamine were treated with B. caapi extract. Subsequently, behavioral tests were conducted, including the elevated plus maze, rotarod, and open field. Tyrosine hydroxylase positive (TH+) neurons and IBA-1 positive microglia were quantified from the LC inflammatory markers and free radical products were assessed.
Results: Both 6-Hydroxydopamine hydrochloride and the Banisteriopsis caapi extract causes reduction of LC neurons, at the concentration and frequency used. The LC depletion and the treatment of B. caapi extract interfere with locomotion. B. caapi extract and the LC lesion increased the number and activation of inflammatory cells, such as microglia. B. caapi extract decreases IL-10 in the hippocampus and BDNF gene expression.
Conclusion: This study suggests that B. caapi extract (at the concentration and frequency used) promotes noradrenergic neuron depletion and creates a proinflammatory environment in the CNS.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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