Characterising the role of enolase in a stable Small Colony Variant of Staphylococcus aureus isolated from a diabetic foot infection patient with osteomyelitis.

Autor: Lee J; Department of Molecular and Biomedical Sciences, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia; Australian Centre for Antimicrobial Resistance Ecology (ACARE), The University of Adelaide, Adelaide, South Australia, Australia; Research Centre for Infectious Diseases (RCID), The University of Adelaide, Adelaide, Australia., Carda-Diéguez M; Department of Health and Genomics, Center for Advanced Research in Public Health, FISABIO Institute, Province of Valencia, Valencia, Spain., Vreugde S; Department of Otolaryngology, Head and Neck Surgery, Basil Hetzel Institute, South Australia, Adelaide, Australia., Cooksley C; Department of Otolaryngology, Head and Neck Surgery, Basil Hetzel Institute, South Australia, Adelaide, Australia., Mashayamombe M; Department of Vascular and Endovascular Surgery, Royal Adelaide Hospital, Adelaide, South Australia, Australia., Dawson J; Department of Vascular and Endovascular Surgery, Royal Adelaide Hospital, Adelaide, South Australia, Australia; Discipline of Surgery, The University of Adelaide, Adelaide, South Australia, Australia; Basil Hetzel Institute for Translational Health Research, The Queen Elizabeth Hospital, Woodville South, South Australia, Australia., Fitridge R; Department of Vascular and Endovascular Surgery, Royal Adelaide Hospital, Adelaide, South Australia, Australia; Discipline of Surgery, The University of Adelaide, Adelaide, South Australia, Australia; Basil Hetzel Institute for Translational Health Research, The Queen Elizabeth Hospital, Woodville South, South Australia, Australia., Mira A; Department of Health and Genomics, Center for Advanced Research in Public Health, FISABIO Institute, Province of Valencia, Valencia, Spain., Zilm PS; Adelaide Dental School, The University of Adelaide, Adelaide, South Australia, Australia., Kidd SP; Department of Molecular and Biomedical Sciences, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia; Australian Centre for Antimicrobial Resistance Ecology (ACARE), The University of Adelaide, Adelaide, South Australia, Australia; Research Centre for Infectious Diseases (RCID), The University of Adelaide, Adelaide, Australia. Electronic address: stephen.kidd@adelaide.edu.au.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Nov; Vol. 196, pp. 106918. Date of Electronic Publication: 2024 Sep 05.
DOI: 10.1016/j.micpath.2024.106918
Abstrakt: The switch to alternate cell types by Staphylococcus aureus creates sub-populations even within an active population, that are highly resilient, tolerant to antibiotics and lack clinical symptoms of infection. These cells present a challenge for clinical treatment where even after initial intervention has seemingly cleared the infection, these alternate cell types persist within tissue to revert and cause disease. Small colony variants (SCV) are a cell type which facilitate persistent infection but clinically isolated SCVs are often unstable in laboratory conditions. We have isolated a pair of S. aureus isolates from an individual patient with osteomyelitis presenting with heterogenous phenotypes; a stable SCV (sSCV) and a SCV that reverts upon laboratory culturing to the usual, active and non-SCV cell type. Thus we are able use this pair to investigate and compare the genetic mechanisms that underlie the clinical variatons of SCV phenotype. The switch to the sSCV phenotype was associated with frameshift mutations in the enolase eno and the histidine kinase arlS. The phenoptye of the sSCV was an impeded growth dependent on amino acid catabolism and modulated biofilm. These mutations present potentially a new molecular mechanism which confer persistence within osteomyelitis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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