The effectiveness of antimicrobial photodynamic therapy on catheter infection model.

Autor: Aysert-Yıldız P; Department of Infectious Diseases and Clinical Microbiology, Gazi University School of Medicine, Ankara, Turkey. Electronic address: pinar_aysert@yahoo.com., Kalkancı A; Department of Medical Microbiology, Gazi University School of Medicine, Ankara, Turkey., Erdoğan M; Department of Medical Microbiology, Gazi University School of Medicine, Ankara, Turkey; Department of Medical Microbiology, Sanko University School of Medicine, Gaziantep, Turkey., Özger HS; Department of Infectious Diseases and Clinical Microbiology, Gazi University School of Medicine, Ankara, Turkey., Öztürk A; Department of Medical Microbiology, Niğde Ömer Halisdemir University School of Medicine, Niğde, Turkey., Güzel-Tunçcan Ö; Department of Infectious Diseases and Clinical Microbiology, Gazi University School of Medicine, Ankara, Turkey., Dizbay M; Department of Infectious Diseases and Clinical Microbiology, Gazi University School of Medicine, Ankara, Turkey., Çağlar K; Department of Medical Microbiology, Gazi University School of Medicine, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Nov; Vol. 260, pp. 113026. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1016/j.jphotobiol.2024.113026
Abstrakt: Background/aim: This experimental study aimed to examine the effectiveness of transdermal antimicrobial photodynamic therapy (APDT) with and without antimicrobial lock therapy (ALT), on catheter biofilms.
Methods: S. epidermidis and C. orthopsilosis biofilms were formed within peripheral venous catheters positioned in the marginal ear veins of New Zealand white rabbits. Biofilm formation was confirmed with scanning electron microscopy in two catheters. 24 catheters with staphylococcal biofilms and 24 with fungal biofilms were treated with APDT, ALT or "APDT plus ALT" for five days. Six catheters were separated as controls. APDT was applied with a red colored LED lamp and methylene blue as the photosensitizer. Vancomycin lock solutions were used as ALT for staphylococcal biofilms and amphotericin B for fungal biofilms. The effect of treatment procedures was evaluated by intraluminal biofilm viability testing based on spectrophotometric evaluation, and a quantitative (OD) value was obtained for each catheter.
Results: The mean OD values obtained by 600 nm spectrophotometric reading at 24 h (biofilm viability) after "ALT", "APDT" and "ALT plus APDT" procedures were 0.363, 0.151 and 0.128 for S. epidermidis and 0.092, 0.104 and 0.227 for C. orthopsilosis, respectively. All these OD values obtained after treatment procedures were lower than controls for both S. epidermidis (OD: 0,802) and C. orthopsilosis (OD: 0,315), although there were large fluctuations in our results.
Conclusions: Our results suggest that transdermal APDT may be an effective method for treating staphylococcal and candida biofilms formed within intravenous catheters in our rabbit ear model. The combined use of APDT and ALT might be beneficial in these staphylococcal biofilms.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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