Target-induced enzymatic cleavage cycle amplification reaction-gated organic photoelectrochemical transistor biosensor for rapid detection of okadaic acid.

Autor: Jiang T; Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, PR China., Ju P; Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, PR China; Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation, North China Sea Marine Forecasting Center of State Oceanic Administration, Qingdao, 266061, PR China. Electronic address: jupeng@fio.org.cn., Bi F; Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation, North China Sea Marine Forecasting Center of State Oceanic Administration, Qingdao, 266061, PR China., Chi J; Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, PR China; College of Chemistry and Chemical Engineering, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, No. 238 Songling Road, Qingdao, 266100, PR China., Wen S; Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, PR China., Jiang F; Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, PR China. Electronic address: jiangfh@fio.org.cn., Chi Z; College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao, 266003, PR China. Electronic address: cz1108@ouc.edu.cn.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2025 Jan 01; Vol. 267, pp. 116745. Date of Electronic Publication: 2024 Sep 04.
DOI: 10.1016/j.bios.2024.116745
Abstrakt: Okadaic acid (OA), a predominant toxic entity in Diarrhetic Shellfish Poisoning (DSP), carries substantial significance for both marine ecosystems and human well-being. The nascent organic photoelectrochemical transistor (OPECT) biosensor has emerged as a promising biometric methodology, poised to offer a fresh realm for the detection of marine biotoxins. In this work, a biosensor utilizing signal amplification based on Cd 0.5 Zn 0.5 S/ZnIn 2 S 4 quantum dots (CZS/ZIS QDs) in OPECT was proposed for OA detection, where ZIS QDs were labeled on aptamer and a substantial quantity of QDs were generated via cyclic shearing facilitated through target-induced Exo I enzyme. Owing to the sensitizing influence of ZIS QDs on CZS, the photoelectric conversion efficiency was augmented, culminating in a notable anodic photocurrent upon exposure to light, thereby inducing a transformation in the channel state of the polymer poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) and consequently producing a remarkable modification in the channel current. The detection limit of the biosensor as low as 12.5 pM and a superior stability and specificity was confirmed, which also showed commendable outcomes in actual samples testing. Consequently, this study not only introduces a novel pathway for swift OA detection, but unveils a novel perspective for future expedited and convenient on-site detection of marine biotoxins.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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