Plant Defensin PgD1 a Biotechnological Alternative Against Plant Pathogens.

Autor: de Bem Matos AC; Programa Multicêntrico de Pós-Graduação Em Bioquímica E Biologia Molecular, Universidade do Estado de Santa Catarina - UDESC, Lages, Santa Catarina, Brasil., Telli EMRP; Biomedicina Universidade Do Planalto Catarinense, Lages, Santa Catarina, Brasil., Camillo LC; Programa Multicêntrico de Pós-Graduação Em Bioquímica E Biologia Molecular, Universidade do Estado de Santa Catarina - UDESC, Lages, Santa Catarina, Brasil., da Silva GF; Programa Multicêntrico de Pós-Graduação Em Bioquímica E Biologia Molecular, Universidade do Estado de Santa Catarina - UDESC, Lages, Santa Catarina, Brasil., Gonçalves MJ; Departamento de P&D, Plant Colab Pesquisa E Desenvolvimento Ltda, Lages, Brazil., Casa R; Departamento de Agronomia, Universidade do Estado de Santa Catarina - UDESC, Lages, Santa Catarina, Brasil., Rufato L; Departamento de Agronomia, Universidade do Estado de Santa Catarina - UDESC, Lages, Santa Catarina, Brasil., de Lourdes Borba Magalhães M; Programa Multicêntrico de Pós-Graduação Em Bioquímica E Biologia Molecular, Universidade do Estado de Santa Catarina - UDESC, Lages, Santa Catarina, Brasil. maria.magalhaes@udesc.br.; Department of Food and Animal Science, State University of Santa Catarina, 2090 Luiz de Camões, Lages, SC, 88520-000, Brazil. maria.magalhaes@udesc.br.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2024 Sep 07. Date of Electronic Publication: 2024 Sep 07.
DOI: 10.1007/s12602-024-10333-0
Abstrakt: Plant defensins are small antimicrobial proteins (AMP) that participate in the immune defense of plants through their antibacterial, antiviral and antifungal activities. PgD1 is a defensin from Picea glauca (Canadian Pine) and has antifungal activity against plant pathogens. This activity positions it as an alternative biotechnological agent to pesticides commonly used against these plant fungi diseases. The present study aimed to recombinantly produce PgD1 in Escherichia coli to characterize its in vitro antifungal potential against different phytopathogens. To achieve this, the coding gene was amplified and cloned into pET30a( +). Recombinant plasmid was subsequently introduced into E. coli for the soluble expression of defensin PgD1. To evaluate the antifungal activity of the expressed protein, the growth inhibition test was used in solid and liquid media for approximately 7 days against significant plant pathogens, that cause significant crop damage including: Botrytis cinerea, Colletotrichum gloeosporioides, Colletotrichum musae, Colletotrichum graminicola and Fusarium oxysporum. Additionally, stability assessments included temperature variation experiments and inhibition tests using dithiothreitol (DTT). The results showed that there was significant inhibition of the fungal species tested when in the presence of PgD1. Furthermore, defensin proved to be resistant to temperature variations and demonstrated that part of its stability is due to its primary structure rich in cysteine ​​residues through the denaturation test with dithiothreitol (DTT) where the antifungal activity of PgD1 defensin was inhibited. These data indicate that recombinant PgD1 could be utilized as a plant protection technology in agriculture.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: