First hip hemiarthroplasty in a Göttingen Minipig; surgical and post-mortem protocol.

Autor: Hartmann KT; Department of Veterinary- and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark. katoh@sund.ku.dk., Odgaard A; Department of Orthopedic Surgery, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Knudsen UK; Department of Orthopedic Surgery, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Aalbæk B; Department of Veterinary- and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark., Kvich L; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.; Center for Surgical Science, Department of Surgery, Zealand University Hospital, Køge, Denmark., Birch JM; Department of Veterinary- and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark., Petersen A; Statens Serum Institut, Artillerivej 5, 2300, Copenhagen S, Denmark., Bjarnsholt T; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Jensen HE; Department of Veterinary- and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark., Jensen LK; Department of Veterinary- and Animal Sciences, University of Copenhagen, Grønnegårdsvej 7, 1870, Frederiksberg C, Denmark.
Jazyk: angličtina
Zdroj: Journal of orthopaedic surgery and research [J Orthop Surg Res] 2024 Sep 06; Vol. 19 (1), pp. 549. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1186/s13018-024-05040-z
Abstrakt: Background: Prosthetic joint infections (PJI) are recalcitrant, hard-to-treat infections and severe complications of joint arthroplasty. Therefore, there is a need to develop new effective treatment strategies, and animal models of high clinical relevance are needed. This study aimed to develop a detailed surgical protocol for hip hemiarthroplasty in Göttingen minipigs and a thorough post-mortem sampling protocol to pave the way for creating a minipig PJI model.
Methods: Three adult female Göttingen minipigs underwent surgery with insertion of a hip hemiarthroplasty, using the anterior approach to the hip joint. After surgery the minipigs were followed closely with daily clinical evaluation and gait scoring. Comprehensive post-mortem analyses were performed with evaluation of macroscopic lesions, microbiology, synovial fluid analysis and histology.
Results: The study resulted in the first Göttingen minipig with hip hemiarthroplasty and identified several points of awareness when inserting a hip prosthesis in minipigs, especially the high risk of joint dislocation. A spontaneous PJI occurred in one of the minipigs, revealing an impaired ability of the immune cells to reach the bacteria at the bone-prosthesis interface.
Conclusion: The present study provides a detailed description of surgical technique and post-mortem sampling and validates the suitability of the hip hemiarthroplasty minipig model for future experimental modeling of PJI.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: