Genome-wide analysis of the passion fruit invertase gene family reveals involvement of PeCWINV5 in hexose accumulation.

Autor: Huang D; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China., Wu B; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China., Chen G; Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, P.R. China., Xing W; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China., Xu Y; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China.; Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan Seed Industry Laboratory, Sanya, Hainan, 572025, P.R. China., Ma F; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China.; Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan Seed Industry Laboratory, Sanya, Hainan, 572025, P.R. China., Li H; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China., Hu W; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China., Huang H; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China., Yang L; Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, P.R. China. yangliutibs@126.com., Song S; Tropical Crops Genetic Resources Institute, National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs / Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province / Germplasm Repository of Passiflora, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, P.R. China. songs@catas.cn.; Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan Seed Industry Laboratory, Sanya, Hainan, 572025, P.R. China. songs@catas.cn.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Sep 06; Vol. 24 (1), pp. 836. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1186/s12870-024-05392-y
Abstrakt: Background: Invertases (INVs) are key enzymes in sugar metabolism, cleaving sucrose into glucose and fructose and playing an important role in plant development and the stress response, however, the INV gene family in passion fruit has not been systematically reported.
Results: In this study, a total of 16 PeINV genes were identified from the passion fruit genome and named according to their subcellular location and chromosome position. These include six cell wall invertase (CWINV) genes, two vacuolar invertase (VINV) genes, and eight neutral/alkaline invertase (N/AINV) genes. The gene structures, phylogenetic tree, and cis-acting elements of PeINV gene family were predicted using bioinformatics methods. Results showed that the upstream promoter region of the PeINV genes contained various response elements; particularly, PeVINV2, PeN/AINV3, PeN/AINV5, PeN/AINV6, PeN/AINV7, and PeN/AINV8 had more response elements. Additionally, the expression profiles of PeINV genes under different abiotic stresses (drought, salt, cold temperature, and high temperature) indicated that PeCWINV5, PeCWINV6, PeVINV1, PeVINV2, PeN/AINV2, PeN/AINV3, PeN/AINV6, and PeN/AINV7 responded significantly to these abiotic stresses, which was consistent with cis-acting element prediction results. Sucrose, glucose, and fructose are main soluble components in passion fruit pulp. The contents of total soluble sugar, hexoses, and sweetness index increased significantly at early stages during fruit ripening. Transcriptome data showed that with an increase in fruit development and maturity, the expression levels of PeCWINV2, PeCWINV5, and PeN/AINV3 exhibited an up-regulated trend, especially for PeCWINV5 which showed highest abundance, this correlated with the accumulation of soluble sugar and sweetness index. Transient overexpression results demonstrated that the contents of fructose, glucose and sucrose increased in the pulp of PeCWINV5 overexpressing fruit. It is speculated that this cell wall invertase gene, PeCWINV5, may play an important role in sucrose unloading and hexose accumulation.
Conclusion: In this study, we systematically identified INV genes in passion fruit for the first time and further investigated their physicochemical properties, evolution, and expression patterns. Furthermore, we screened out a key candidate gene involved in hexose accumulation. This study lays a foundation for further study on INV genes and will be beneficial on the genetic improvement of passion fruit breeding.
(© 2024. The Author(s).)
Databáze: MEDLINE
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