Continental-scale associations of Arabidopsis thaliana phyllosphere members with host genotype and drought.

Autor: Karasov TL; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA. t.karasov@utah.edu.; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany. t.karasov@utah.edu., Neumann M; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany.; Robert Bosch GmbH, Renningen, Germany., Leventhal L; Department of Biology, Stanford University, Stanford, CA, USA.; Department of Plant Biology, Carnegie Institution for Plant Science, Stanford, CA, USA., Symeonidi E; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA., Shirsekar G; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany.; Department of Entomology and Plant Pathology, Institute of Agriculture, University of Tennessee, Knoxville, TN, USA., Hawks A; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA., Monroe G; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany.; Department of Plant Sciences, University of California Davis, Davis, CA, USA., Exposito-Alonso M; Department of Biology, Stanford University, Stanford, CA, USA.; Department of Plant Biology, Carnegie Institution for Plant Science, Stanford, CA, USA.; Department of Integrative Biology, University of California Berkeley, Berkeley, CA, USA.; Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA, USA., Bergelson J; Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, USA., Weigel D; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany. weigel@tue.mpg.de.; Institute for Bioinformatics and Medical Informatics, University of Tübingen, Tübingen, Germany. weigel@tue.mpg.de., Schwab R; Department of Molecular Biology, Max Planck Institute for Biology Tübingen, Tübingen, Germany.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2024 Sep 06. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1038/s41564-024-01773-z
Abstrakt: Plants are colonized by distinct pathogenic and commensal microbiomes across different regions of the globe, but the factors driving their geographic variation are largely unknown. Here, using 16S ribosomal DNA and shotgun sequencing, we characterized the associations of the Arabidopsis thaliana leaf microbiome with host genetics and climate variables from 267 populations in the species' native range across Europe. Comparing the distribution of the 575 major bacterial amplicon variants (phylotypes), we discovered that microbiome composition in A. thaliana segregates along a latitudinal gradient. The latitudinal clines in microbiome composition are predicted by metrics of drought, but also by the spatial genetics of the host. To validate the relative effects of drought and host genotype we conducted a common garden field study, finding 10% of the core bacteria to be affected directly by drought and 20% to be affected by host genetic associations with drought. These data provide a valuable resource for the plant microbiome field, with the identified associations suggesting that drought can directly and indirectly shape genetic variation in A. thaliana via the leaf microbiome.
(© 2024. The Author(s).)
Databáze: MEDLINE
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