High-quality identification of volatile organic compounds (VOCs) originating from breath.

Autor: Arulvasan W; Owlstone Medical Ltd, Cambridge, UK. wisenave.arulvasan@owlstone.co.uk., Chou H; Owlstone Medical Ltd, Cambridge, UK., Greenwood J; Owlstone Medical Ltd, Cambridge, UK., Ball ML; Owlstone Medical Ltd, Cambridge, UK., Birch O; Owlstone Medical Ltd, Cambridge, UK., Coplowe S; Owlstone Medical Ltd, Cambridge, UK., Gordon P; Owlstone Medical Ltd, Cambridge, UK., Ratiu A; Owlstone Medical Ltd, Cambridge, UK., Lam E; Owlstone Medical Ltd, Cambridge, UK., Hatch A; Owlstone Medical Ltd, Cambridge, UK., Szkatulska M; Owlstone Medical Ltd, Cambridge, UK., Levett S; Owlstone Medical Ltd, Cambridge, UK., Mead E; Owlstone Medical Ltd, Cambridge, UK., Charlton-Peel C; Owlstone Medical Ltd, Cambridge, UK., Nicholson-Scott L; Owlstone Medical Ltd, Cambridge, UK., Swann S; Owlstone Medical Ltd, Cambridge, UK., van Schooten FJ; Faculty of Health, Medicine and Life Sciences, Pharmacology and Toxicology, Maastricht University, Maastricht, Netherlands., Boyle B; Owlstone Medical Ltd, Cambridge, UK., Allsworth M; Owlstone Medical Ltd, Cambridge, UK.
Jazyk: angličtina
Zdroj: Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2024 Sep 06; Vol. 20 (5), pp. 102. Date of Electronic Publication: 2024 Sep 06.
DOI: 10.1007/s11306-024-02163-6
Abstrakt: Introduction: Volatile organic compounds (VOCs) can arise from underlying metabolism and are detectable in exhaled breath, therefore offer a promising route to non-invasive diagnostics. Robust, precise, and repeatable breath measurement platforms able to identify VOCs in breath distinguishable from background contaminants are needed for the confident discovery of breath-based biomarkers.
Objectives: To build a reliable breath collection and analysis method that can produce a comprehensive list of known VOCs in the breath of a heterogeneous human population.
Methods: The analysis cohort consisted of 90 pairs of breath and background samples collected from a heterogenous population. Owlstone Medical's Breath Biopsy ® OMNI ® platform, consisting of sample collection, TD-GC-MS analysis and feature extraction was utilized. VOCs were determined to be "on-breath" if they met at least one of three pre-defined metrics compared to paired background samples. On-breath VOCs were identified via comparison against purified chemical standards, using retention indexing and high-resolution accurate mass spectral matching.
Results: 1471 VOCs were present in > 80% of samples (breath and background), and 585 were on-breath by at least one metric. Of these, 148 have been identified covering a broad range of chemical classes.
Conclusions: A robust breath collection and relative-quantitative analysis method has been developed, producing a list of 148 on-breath VOCs, identified using purified chemical standards in a heterogenous population. Providing confirmed VOC identities that are genuinely breath-borne will facilitate future biomarker discovery and subsequent biomarker validation in clinical studies. Additionally, this list of VOCs can be used to facilitate cross-study data comparisons for improved standardization.
(© 2024. The Author(s).)
Databáze: MEDLINE
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