Characterization of the membrane vesicle fraction from Acetobacter sp. WSS15.

Autor: Kurata A; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan. Electronic address: kurata090401@nara.kindai.ac.jp., Aimatsu K; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan., Kimura Y; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan., Hashiguchi H; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan., Maeda A; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan., Imai T; Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan., Yamasaki-Yashiki S; Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan., Hamada K; Sakamoto Kurozu, Inc., 21-15 Uenosono-cho, Kagoshima, Kagoshima 890-0052, Japan., Fujimoto Y; Sakamoto Kurozu, Inc., 21-15 Uenosono-cho, Kagoshima, Kagoshima 890-0052, Japan., Fujii A; Sakamoto Kurozu, Inc., 21-15 Uenosono-cho, Kagoshima, Kagoshima 890-0052, Japan., Uegaki K; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan; Agricultural Technology and Innovation Research Institute, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan.
Jazyk: angličtina
Zdroj: Journal of bioscience and bioengineering [J Biosci Bioeng] 2024 Dec; Vol. 138 (6), pp. 495-500. Date of Electronic Publication: 2024 Sep 05.
DOI: 10.1016/j.jbiosc.2024.07.017
Abstrakt: A bacterium that produces membrane vesicles (MVs), strain WSS15, was isolated from a traditional vinegar in Japan called Kurozu. A phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium belongs to the genus Acetobacter. MVs and peptidoglycan-associated lipoprotein (Pal) were detected in the MV fraction of strain WSS15. In the presence of the WSS15 MV fraction, murine macrophages produced the pro-inflammatory cytokine interleukin-6 (IL-6) via the recognition by superficial Toll-like receptor 2 (TLR2). WSS15 MVs adhered to the cell surface of macrophages. The macrophages secreted IL-6 through the TLR2 recognition of an acylated N-terminal peptide of Pal. We elucidated the mode of action of WSS15 MVs on immune cells and identified the Pal peptide from strain WSS15 as an agonist of TLR2.
(Copyright © 2024 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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