Investigation of the concentration and isotopic composition of Cu, Fe and Zn in human biofluids in the context of Alzheimer's disease via tandem and multi-collector inductively coupled plasma-mass spectrometry.

Autor: Hobin K; Atomic & Mass Spectrometry - A&MS Research Unit, Department of Chemistry, Ghent University, Campus Sterre, Krijgslaan 281 - S12, Ghent 9000, Belgium., Abou-Zeid L; Atomic & Mass Spectrometry - A&MS Research Unit, Department of Chemistry, Ghent University, Campus Sterre, Krijgslaan 281 - S12, Ghent 9000, Belgium., Mendizabal IB; Atomic & Mass Spectrometry - A&MS Research Unit, Department of Chemistry, Ghent University, Campus Sterre, Krijgslaan 281 - S12, Ghent 9000, Belgium., Van Vrekhem T; Cognitive Center, Department of Neurology, Ghent University Hospital, Corneel Heymanslaan 10, Ghent 9000, Belgium., Miatton M; Cognitive Center, Department of Neurology, Ghent University Hospital, Corneel Heymanslaan 10, Ghent 9000, Belgium., D'Haeze B; Cognitive Center, Department of Neurology, Ghent University Hospital, Corneel Heymanslaan 10, Ghent 9000, Belgium., Scarioni M; Cognitive Center, Department of Neurology, Ghent University Hospital, Corneel Heymanslaan 10, Ghent 9000, Belgium., Van Langenhove T; Cognitive Center, Department of Neurology, Ghent University Hospital, Corneel Heymanslaan 10, Ghent 9000, Belgium., Vanhaecke F; Atomic & Mass Spectrometry - A&MS Research Unit, Department of Chemistry, Ghent University, Campus Sterre, Krijgslaan 281 - S12, Ghent 9000, Belgium. Electronic address: frank.vanhaecke@ugent.be.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2024 Aug 30; Vol. 86, pp. 127515. Date of Electronic Publication: 2024 Aug 30.
DOI: 10.1016/j.jtemb.2024.127515
Abstrakt: Studies on essential trace elements in the context of Alzheimer's disease (AD) concluded that Cu, Fe and Zn interact with amyloid-β, accelerating plaque formation in the brain. Additionally, Cu and Fe in the vicinity of plaques produce reactive oxygen species (ROS) resulting in oxidative stress, whereas Zn plays a role in the antioxidant defence as a co-factor for antioxidants. In this work, the Cu, Fe and Zn concentrations and isotope ratios were determined in whole blood, blood serum and cerebrospinal fluid of 10 patients diagnosed with AD and 8 control individuals, using tandem (ICP-MS/MS) and multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS), respectively. In whole blood and blood serum of AD patients, a heavier Cu isotopic composition was observed (significant for whole blood only) compared to controls. Albumin levels in cerebrospinal fluid tend to increase with age, which could indicate an increased leakiness of the blood-brain barrier. In cerebrospinal fluid, a large variability was observed for the Cu and Fe isotope ratios, potentially resulting from that leakiness at the blood-brain barrier. Therefore, potential effects of AD on the concentration and isotopic composition of essential elements in cerebrospinal fluid related to amyloid-β formation could be hidden. Finally, in blood serum, Zn, urea and creatinine concentrations showed an increase with age and showed a significant difference between sexes.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
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Databáze: MEDLINE
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