Comparison of next generation sequencing (NGS) - (SNPs) and capillary electrophoresis (CE) - (STRs) in the genetic analysis of human remains.

Autor: Kokotas S; Department of Medical Research, 251 Hellenic Air Force & VA General Hospital, Athens, Greece., Budowle B; Department of Forensic Medicine, University of Helsinki, Helsinki, Finland; Forensic Science Institute, Radford University, Radford, VA, USA., Papatheodorou A; Department of Medical Research, 251 Hellenic Air Force & VA General Hospital, Athens, Greece., Bolanaki E; Department of Medical Research, 251 Hellenic Air Force & VA General Hospital, Athens, Greece., Kondili A; Forensic Science Division, Hellenic Police, Athens, Greece., Metheniti A; Forensic Science Division, Hellenic Police, Athens, Greece., Vouropoulou M; Forensic Science Division, Hellenic Police, Athens, Greece., Koukouvinos G; Forensic Science Division, Hellenic Police, Athens, Greece., Palaigeorgiou E; Forensic Science Division, Hellenic Police, Athens, Greece., Makras P; Department of Medical Research, 251 Hellenic Air Force & VA General Hospital, Athens, Greece. Electronic address: pmakras@gmail.com.
Jazyk: angličtina
Zdroj: Forensic science international. Genetics [Forensic Sci Int Genet] 2025 Jan; Vol. 74, pp. 103131. Date of Electronic Publication: 2024 Aug 30.
DOI: 10.1016/j.fsigen.2024.103131
Abstrakt: A pilot study was performed using two different DNA technology platforms conducted by two laboratories to analyze DNA extracted from 83-year-old, human male skeletal remains from 16 individuals, of which there are no other viable means to identify these war victims. The workflow of the more recent developed ForenSeq Kintelligence Kit and next generation sequencing was compared to that of the standard capillary electrophoresis - short tandem repeat (STR) method (Power Plex ESX17 and Y23 Systems). The findings indicate that greater amount of useful genetic data can be gained with the Kintelligence system across the range of samples under study and particularly for samples in which partial or no STR profiles are obtained. SNP data are more likely to be obtained from degraded samples, like the ones analyzed in this study. Moreover, high volume SNP data are suitable for long distance kinship associations and genetic genealogy databases to develop more investigative leads for future kinship and missing persons cases, a process not feasible by STR typing.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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