An integrative taxonomy approach reveals Saccharomyces chiloensis sp. nov. as a newly discovered species from Coastal Patagonia.

Autor: Peña TA; Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile.; Millennium Institute for Integrative Biology (iBio), Santiago, Chile., Villarreal P; Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile.; Millennium Institute for Integrative Biology (iBio), Santiago, Chile., Agier N; Laboratory of Computational and Quantitative Biology, CNRS, Institut de Biologie Paris-Seine, Sorbonne Université, Paris, France., De Chiara M; Université Côte d'Azur, CNRS, INSERM, IRCAN, Nice, France., Barría T; Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile., Urbina K; Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile.; Millenium Nucleus of Patagonian Limit of Life (LiLi), Santiago, Chile., Villarroel CA; Centro de Biotecnología de los Recursos Naturales (CENBio), Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Talca, Chile., Santos ARO; Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Rosa CA; Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Nespolo RF; Millennium Institute for Integrative Biology (iBio), Santiago, Chile.; Millenium Nucleus of Patagonian Limit of Life (LiLi), Santiago, Chile.; Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile, Valdivia, Chile.; Center of Applied Ecology and Sustainability (CAPES), Facultad de Ciencias Biológicas, Universidad Católica de Chile, Santiago, Chile., Liti G; Université Côte d'Azur, CNRS, INSERM, IRCAN, Nice, France., Fischer G; Laboratory of Computational and Quantitative Biology, CNRS, Institut de Biologie Paris-Seine, Sorbonne Université, Paris, France., Cubillos FA; Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, Santiago, Chile.; Millennium Institute for Integrative Biology (iBio), Santiago, Chile.; Millenium Nucleus of Patagonian Limit of Life (LiLi), Santiago, Chile.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2024 Sep 06; Vol. 20 (9), pp. e1011396. Date of Electronic Publication: 2024 Sep 06 (Print Publication: 2024).
DOI: 10.1371/journal.pgen.1011396
Abstrakt: Species delineation in microorganisms is challenging due to the limited markers available for accurate species assignment. Here, we applied an integrative taxonomy approach, combining extensive sampling, whole-genome sequence-based classification, phenotypic profiling, and assessment of interspecific reproductive isolation. Our work reveals the presence of a distinct Saccharomyces lineage in Nothofagus forests of coastal Patagonia. This lineage, designated Saccharomyces chiloensis sp. nov., exhibits 7% genetic divergence from its sister species S. uvarum, as revealed by whole-genome sequencing and population analyses. The South America-C (SA-C) coastal Patagonia population forms a unique clade closely related to a previously described divergent S. uvarum population from Oceania (AUS, found in Australia and New Zealand). Our species reclassification is supported by a low Ortho Average Nucleotide Identity (OANI) of 93% in SA-C and AUS relative to S. uvarum, which falls below the suggested species delineation threshold of 95%, indicating an independent evolutionary lineage. Hybrid spore viability assessment provided compelling evidence that SA-C and AUS are reproductively isolated from S. uvarum. In addition, we found unique structural variants between S. chiloensis sp. nov. lineages, including large-scale chromosomal translocations and inversions, together with a distinct phenotypic profile, emphasizing their intraspecies genetic distinctiveness. We suggest that S. chiloensis sp. nov diverged from S. uvarum in allopatry due to glaciation, followed by post-glacial dispersal, resulting in distinct lineages on opposite sides of the Pacific Ocean. The discovery of S. chiloensis sp. nov. illustrates the uniqueness of Patagonia's coastal biodiversity and underscores the importance of adopting an integrative taxonomic approach in species delineation to unveil cryptic microbial species. The holotype of S. chiloensis sp. nov. is CBS 18620T.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Peña et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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