RluA is the major mRNA pseudouridine synthase in Escherichia coli.

Autor: Schaening-Burgos C; Department of Biology, Massachusetts Institute of Technology; Cambridge, Massachusetts, United States of America.; Program in Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America., LeBlanc H; Department of Biology, Massachusetts Institute of Technology; Cambridge, Massachusetts, United States of America., Fagre C; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America., Li GW; Department of Biology, Massachusetts Institute of Technology; Cambridge, Massachusetts, United States of America., Gilbert WV; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2024 Sep 06; Vol. 20 (9), pp. e1011100. Date of Electronic Publication: 2024 Sep 06 (Print Publication: 2024).
DOI: 10.1371/journal.pgen.1011100
Abstrakt: Pseudouridine (Ψ) is an ubiquitous RNA modification, present in the tRNAs and rRNAs of species across all domains of life. Conserved pseudouridine synthases modify the mRNAs of diverse eukaryotes, but the modification has yet to be identified in bacterial mRNAs. Here, we report the discovery of pseudouridines in mRNA from E. coli. By testing the mRNA modification capacity of all 11 known pseudouridine synthases, we identify RluA as the predominant mRNA-modifying enzyme. RluA, a known tRNA and 23S rRNA pseudouridine synthase, modifies at least 31 of the 44 high-confidence sites we identified in E. coli mRNAs. Using RNA structure probing data to inform secondary structures, we show that the target sites of RluA occur in a common sequence and structural motif comprised of a ΨURAA sequence located in the loop of a short hairpin. This recognition element is shared with previously identified target sites of RluA in tRNAs and rRNA. Overall, our work identifies pseudouridine in key mRNAs and suggests the capacity of Ψ to regulate the transcripts that contain it.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Schaening-Burgos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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