A comprehensive in silico investigation into the pathogenic SNPs in the RTEL1 gene and their biological consequences.

Autor: Tanshee RR; Department of Mathematics and Natural Sciences, BRAC University, Badda, Dhaka, Bangladesh., Mahmud Z; Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh., Nabi AHMN; Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh., Sayem M; Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Sep 06; Vol. 19 (9), pp. e0309713. Date of Electronic Publication: 2024 Sep 06 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0309713
Abstrakt: The Regulator of Telomere Helicase 1 (RTEL1) gene encodes a critical DNA helicase intricately involved in the maintenance of telomeric structures and the preservation of genomic stability. Germline mutations in the RTEL1 gene have been clinically associated with Hoyeraal-Hreidarsson syndrome, a more severe version of Dyskeratosis Congenita. Although various research has sought to link RTEL1 mutations to specific disorders, no comprehensive investigation has yet been conducted on missense mutations. In this study, we attempted to investigate the functionally and structurally deleterious coding and non-coding SNPs of the RTEL1 gene using an in silico approach. Initially, out of 1392 nsSNPs, 43 nsSNPs were filtered out through ten web-based bioinformatics tools. With subsequent analysis using nine in silico tools, these 43 nsSNPs were further shortened to 11 most deleterious nsSNPs. Furthermore, analyses of mutated protein structures, evolutionary conservancy, surface accessibility, domains & PTM sites, cancer susceptibility, and interatomic interaction revealed the detrimental effect of these 11 nsSNPs on RTEL1 protein. An in-depth investigation through molecular docking with the DNA binding sequence demonstrated a striking change in the interaction pattern for F15L, M25V, and G706R mutant proteins, suggesting the more severe consequences of these mutations on protein structure and functionality. Among the non-coding variants, two had the highest likelihood of being regulatory variants, whereas one variant was predicted to affect the target region of a miRNA. Thus, this study lays the groundwork for extensive analysis of RTEL1 gene variants in the future, along with the advancement of precision medicine and other treatment modalities.
Competing Interests: The authors report that there are no competing interests to declare.
(Copyright: © 2024 Tanshee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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