Comparison of Immune Checkpoint Molecule Expression in Different Years of House Dust Mite Subcutaneous Immunotherapy on CD4 + T and Treg Cells in Children with Allergic Rhinitis.
| Autor: | Hızlı Demirkale Z; Department of Immunology, İstanbul University, Aziz Sancar Institute of Experimental Medicine, İstanbul, Türkiye.; İstanbul University, Institute of Graduate Studies in Health Sciences, İstanbul, Türkiye.; Department of Pediatrics, Division of Pediatric Allergy and Immunology, İstanbul University, İstanbul Faculty of Medicine, İstanbul, Türkiye., Alpkıray MF; Department of Pediatrics, İstanbul University, İstanbul Faculty of Medicine, İstanbul, Türkiye., Engin A; Department of Immunology, İstanbul University, Aziz Sancar Institute of Experimental Medicine, İstanbul, Türkiye., Sönmez AD; Department of Immunology, İstanbul University, Aziz Sancar Institute of Experimental Medicine, İstanbul, Türkiye., Yücel E; Department of Pediatrics, Division of Pediatric Allergy and Immunology, İstanbul University, İstanbul Faculty of Medicine, İstanbul, Türkiye., Tamay Z; Department of Pediatrics, Division of Pediatric Allergy and Immunology, İstanbul University, İstanbul Faculty of Medicine, İstanbul, Türkiye., Özdemir C; Department of Pediatrics, Division of Pediatric Allergy and Immunology, İstanbul University, İstanbul Faculty of Medicine, İstanbul, Türkiye.; Department of Pediatric Basic Sciences, İstanbul University, Institute of Child Health, İstanbul, Türkiye., Deniz G; Department of Immunology, İstanbul University, Aziz Sancar Institute of Experimental Medicine, İstanbul, Türkiye., Çetin Aktaş E; Department of Immunology, İstanbul University, Aziz Sancar Institute of Experimental Medicine, İstanbul, Türkiye. |
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| Jazyk: | angličtina |
| Zdroj: | Balkan medical journal [Balkan Med J] 2024 Sep 06; Vol. 41 (5), pp. 387-395. |
| DOI: | 10.4274/balkanmedj.galenos.2024.2024-6-19 |
| Abstrakt: | Background: Allergen-specific immunotherapy, a unique inducer of tolerance, may result in T cell exhaution. Aims: To investigate how the duration of house dust mite (HDM) subcutaneous immunotherapy (SCIT) affects the expression of major immune checkpoint (ICP) molecules on the surface of CD4 + T-helper and regulatory T (Treg) cells. Study Design: Cross-sectional study. Methods: We enrolled 28 children with HDM-induced allergic rhinitis (AR) and six controls. The study participants were divided into six groups: one group each of patients in their first, second, and third years of HDM-SCIT; one group each comprising those in the first year following HDM-SCIT and those on pharmacotherapy; and the control group. The expression of ICPs on CD4 + T and Treg cells was determined using flow cytometry, and plasma levels of soluble ICPs were estimated by ELISA. Results: Our results revealed a significant increase in the expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and lymphocyte activation gene 3 (LAG-3) on CD4 + T cells during the second and third years of SCIT, respectively. Additionally, a strong correlation was observed between the expression of CTLA-4 and T cell immunoglobulin and mucin domain containing molecule-3 in CD4 + T cells. Furthermore, we observed a significant correlation between the expressions of programmed cell death protein-1, CTLA-4, T cell Immunoreceptor with Immunoglobulin and Immunoreceptor Tyrosine-Based Inhibitory Motif domain, and LAG-3 on both CD4 + T and Treg cells. A robust correlation was observed between the plasma levels of soluble ICPs. Conclusion: HDM-SCIT induces CD4 + T cell exhaution, which may contribute to tolerance induction in children with AR. Competing Interests: Conflict of Interest: The authors declare that they have no conflict of interest. |
| Databáze: | MEDLINE |
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