Human tetraspanin CD81 facilitates invasion of Salmonella enterica into human epithelial cells.

Autor: Alvarez KG; Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, Germany., Goral L; Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, Germany., Suwandi A; Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, Germany.; Institute of Cell Biochemistry, Hannover Medical School, Hannover, Germany., Lasswitz L; Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), Tierärztliche Hochschule Hannover, Hannover, Germany., Zapatero-Belinchón FJ; Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), Tierärztliche Hochschule Hannover, Hannover, Germany., Ehrhardt K; Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, Germany., Nagarathinam K; Institute for Biochemistry, Universität zu Lübeck, Lübeck, Germany.; Excellence Cluster 2155 RESIST, Medizinische Hochschule Hannover, Hannover, Germany., Künnemann K; Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, Germany., Krey T; Institute for Biochemistry, Universität zu Lübeck, Lübeck, Germany.; Excellence Cluster 2155 RESIST, Medizinische Hochschule Hannover, Hannover, Germany.; Institute of Virology, Medizinische Hochschule Hannover, Hannover, Germany.; German Centre for Infection Research (DZIF), Lübeck, Germany.; Centre for Structural Systems Biology (CSSB), Hamburg, Germany., Wiedemann A; IRSD - Institut de Recherche en Santé Digestive, ENVT, INRAE, INSERM, Université́ de Toulouse, UPS, Toulouse, France., Gerold G; Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), Tierärztliche Hochschule Hannover, Hannover, Germany.; Department of Clinical Microbiology, Umeå University, Umeå, Sweden.; Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, Sweden., Grassl GA; Institute of Medical Microbiology and Hospital Epidemiology, Medizinische Hochschule Hannover, Germany.; German Centre for Infection Research (DZIF), Hannover, Germany.
Jazyk: angličtina
Zdroj: Virulence [Virulence] 2024 Dec; Vol. 15 (1), pp. 2399792. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1080/21505594.2024.2399792
Abstrakt: Human CD81 and CD9 are members of the tetraspanin family of proteins characterized by a canonical structure of four transmembrane domains and two extracellular loop domains. Tetraspanins are known as molecular facilitators, which assemble and organize cell surface receptors and partner molecules forming clusters known as tetraspanin-enriched microdomains. They have been implicated to play various biological roles including an involvement in infections with microbial pathogens. Here, we demonstrate an important role of CD81 for the invasion of epithelial cells by Salmonella enterica . We show that the overexpression of CD81 in HepG2 cells enhances invasion of various typhoidal and non-typhoidal Salmonella serovars. Deletion of CD81 by CRISPR/Cas9 in intestinal epithelial cells (C2BBe1 and HT29-MTX-E12) reduces S . Typhimurium invasion. In addition, the effect of human CD81 is species-specific as only human but not rat CD81 facilitates Salmonella invasion. Finally, immunofluorescence microscopy and proximity ligation assay revealed that both human tetraspanins CD81 and CD9 are recruited to the entry site of S . Typhimurium during invasion but not during adhesion to the host cell surface. Overall, we demonstrate that the human tetraspanin CD81 facilitates Salmonella invasion into epithelial host cells.
Databáze: MEDLINE
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