Loop-mediated isothermal amplification (LAMP) for detection of atypical enterovirus D68 strain VR-1197.

Autor: Tveten AK; Faculty of Natural Sciences, Department of Biological Sciences Ålesund, Norwegian University of Science and Technology (NTNU), Larsgaardsveien 2, Aalesund NO-6009, Norway. Electronic address: ann-kristin.tveten@ntnu.no., Ørstenvik HL; Faculty of Natural Sciences, Department of Biological Sciences Ålesund, Norwegian University of Science and Technology (NTNU), Larsgaardsveien 2, Aalesund NO-6009, Norway., Tolaas I; Faculty of Natural Sciences, Department of Biological Sciences Ålesund, Norwegian University of Science and Technology (NTNU), Larsgaardsveien 2, Aalesund NO-6009, Norway.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 Dec; Vol. 330, pp. 115030. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1016/j.jviromet.2024.115030
Abstrakt: A method that has rapidly evolved for detection of viral pathogens are loop-mediated isothermal amplification (LAMP) assays. The available LAMP assays usually target the most common viral strains, including enteroviruses, but for the atypical enterovirus D68 strain VR-1197 this method has not yet been developed. Enterovirus D68 are known for severe respiratory distress in children, and atypical strains are less likely to be detected by traditional methods. This study targets the atypical EVD68 strain VR-1197 and have developed a rapid detection method saving time when differentiating enterovirus strains. This study present method development and review the sensitivity and specificity compared to traditional RT-qPCR, and wet lab cross reactivity with other airway pathogens. The EVD68 VR-1197 assay can be a rapid POC (Point of care) test for atypical EVD68 VR-1197 and have the potential as reliable detection method with minimal technological requirements.
Competing Interests: Declaration of Competing Interest Authors declare that they have no competing interests.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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