RpoS sigma factor mediates adaptation and virulence in Vibrio mimicus.

Autor: Jiang Z; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Chen A; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Chen Z; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Xu J; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Gao X; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Jiang Q; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Zhang X; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. Electronic address: zxj9307@163.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 279 (Pt 3), pp. 135307. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1016/j.ijbiomac.2024.135307
Abstrakt: The alternative sigma factor RpoS functions as a regulator of stress and virulence response in numerous bacterial species. Vibrio mimicus is a critical opportunistic pathogen causing huge losses to aquaculture. However, the exact role of RpoS in V. mimicus remains unclear. In this study, rpoS deletion mutant of V. mimicus was constructed through allelic exchange and the phenotypic and transcriptional changes were investigated to determine the function of RpoS. The abilities of growth, motility, biofilm production, hemolytic activity and pathogenicity were significantly impaired in ΔrpoS strain. Stationary-phase cells of ΔrpoS strain showed lower tolerance to H 2 O 2 , heat, ethanol, and starvation stress than the wild-type strain. Transcriptome analyses revealed the involvement of rpoS in various cellular processes, notably bacterial-type flagellum synthesis and assembly, membrane synthesis and assembly and response to various stimuli. Phenotypic and RNA-seq analysis revealed that RpoS is required for biofilm formation, stress resistance, and pathogenicity in V. mimicus. Furthermore, β-galactosidase activity showed that rpoS is essential for optimal transcription of the flgK, fliA, cheA, mcpH mRNA. These results offer significant insight into the function and regulatory network of rpoS/RpoS, thereby improving our understanding and facilitating selection of molecular targets for future prevention strategies against V. mimicus.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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