Biochar and arbuscular mycorrhizal fungi promote rapid-cycling Brassica napus growth under cadmium stress.

Autor: Yin C; Center of Space Exploration, Ministry of Education, Chongqing University, Chongqing 400044, China; School of Life Sciences, Chongqing University, Chongqing 401331, China; Chongqing Research Institute of NCU, Chongqing 402660, China., Lei W; Crop Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China., Wang S; Center of Space Exploration, Ministry of Education, Chongqing University, Chongqing 400044, China; School of Life Sciences, Chongqing University, Chongqing 401331, China; Chongqing Research Institute of NCU, Chongqing 402660, China., Xie G; Center of Space Exploration, Ministry of Education, Chongqing University, Chongqing 400044, China; Chongqing Research Institute of NCU, Chongqing 402660, China; Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400044, China. Electronic address: xiegengxin@vip.sina.com., Qiu D; Center of Space Exploration, Ministry of Education, Chongqing University, Chongqing 400044, China; School of Life Sciences, Chongqing University, Chongqing 401331, China; Chongqing Research Institute of NCU, Chongqing 402660, China. Electronic address: qiudan@cqu.edu.cn.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Nov 25; Vol. 953, pp. 176034. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1016/j.scitotenv.2024.176034
Abstrakt: Purpose: To explore the mechanisms of tolerance of Brassica napus to ultra-high concentration cadmium pollution and the synergistic effects of biochar (BC) and Arbuscular mycorrhizal fungi (AMF) on plant growth under cadmium (Cd) stress.
Results: The application of 5 % BC and inoculation with 10 g AMF significantly promoted the growth and development of B. napus. The combined application of BC and AMF (BC1A and BC2A) was better than the single application. At the Cd 200 mg/kg level, BC1A increased the fresh weight and Cd content of the above-ground parts of B. napus by 35.5 % and decreased by 21.20 %. The SOD and POD activities increased by 30.63 % and 73.37 %. The MDA and H 2 O 2 contents decreased by 40.8 % and 69.99 %, soluble sugar content increased by 37.96 %. At the Cd 300 mg/kg level, BC1A increased the fresh weight and Cd content of the above-ground parts of B. napus by 32.8 % and decreased by 15.99 %. The SOD and POD activities increased by 39.06 % and 93.56 %. The MDA and H 2 O 2 contents decreased by 28.39 % and 72.45 %, and the soluble sugar content increased by 21.16 %. Overall, both BC and AMF treatments alone or in combination (BC1A) were able to alleviate Cd stress and promote plant growth, with the combination of biochar and AMF being the most effective. Furthermore, transcriptome analyses indicated that BC may improve cadmium resistance in B. napus by significantly up-regulating the expression of genes related to peroxidase, photosynthesis, and plant MAPK signaling pathways. AMF may alleviate the toxicity of Cd stress on B. napus by up-regulating the expression of genes related to peroxisomes, phytohormone signaling, and carotenoid biosynthesis. The results of the study will provide support for ecological restoration technology in extremely heavy metal-polluted environments and provide some reference for the application and popularization of BC and AMF conjugation technology.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dan Qiu reports financial support was provided by Chongqing University. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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