Genetic traits and transmission of antimicrobial resistance characteristics of cephalosporin resistant Escherichia coli in tropical aquatic environments.

Autor: Yuan Q; Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore. Electronic address: e0638376@u.nus.edu., Li W; NUS Environmental Research Institute, National University of Singapore, Singapore 117411, Singapore; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: li_wenxuan14@u.nus.edu., Goh SG; NUS Environmental Research Institute, National University of Singapore, Singapore 117411, Singapore. Electronic address: erigsg@nus.edu.sg., Chen SL; Genome Institute of Singapore, Singapore 138672, Singapore. Electronic address: slchen@gis.a-star.edu.sg., Ng OT; National Centre for Infectious Diseases, Singapore 308442, Singapore. Electronic address: oon_tek_ng@ncid.sg., He Y; School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: ylhe@sjtu.edu.cn., Gin KY; Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore; NUS Environmental Research Institute, National University of Singapore, Singapore 117411, Singapore. Electronic address: ceeginyh@nus.edu.sg.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Nov 05; Vol. 479, pp. 135707. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1016/j.jhazmat.2024.135707
Abstrakt: This study investigates the genetic traits and transmission mechanisms of cephalosporin-resistant Escherichia coli in tropical aquatic environments in Singapore. From 2016 to 2020, monthly samples were collected from wastewater treatment plants, marine niches, community sewage, beaches, reservoirs, aquaculture farms, and hospitals, yielding 557 isolates that were analyzed for antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) using genomic methods. Findings reveal significant genotypic similarities between environmental and hospital-derived strains, particularly the pandemic E. coli ST131. Environmental strains exhibited high levels of intrinsic resistance mechanisms, including mutations in porins and efflux pumps, with key ARGs such as CMY-2 and NDM-9 predominantly carried by MGEs, which facilitate horizontal gene transfer. Notably, pathogenic EPEC and EHEC strains were detected in community sewage and aquaculture farms, posing substantial public health risks. This underscores the critical role of these environments as reservoirs for multidrug-resistant pathogens and emphasizes the interconnectedness of human activities and environmental health.
Competing Interests: Declaration of Competing Interest We confirmed that there are no competing interests in this study.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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