Enrichment of antioxidant peptide from rice protein hydrolysates via rice husk derived biochar.

Autor: Tao HZ; School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China., He WB; Hunan Provincial Institute of Product and Goods Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan, China 410007., Ding L; School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China., Wen L; School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China., Xu Z; School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China., Cheng YH; School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China. Electronic address: cyh@csust.edu.cn., Chen ML; School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan 410114, China. Electronic address: mlchen@xmu.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2025 Jan 15; Vol. 463 (Pt 1), pp. 141050. Date of Electronic Publication: 2024 Aug 30.
DOI: 10.1016/j.foodchem.2024.141050
Abstrakt: In this study, rice husk biochar was engineered with abundant iron ion sites to enhance the enrichment of antioxidant peptides from rice protein hydrolysates through metal-chelating interactions. The π-π interactions and metal ion chelation were identified as the primary mechanisms for the enrichment process. Through peptide sequencing, four peptides were identified: LKFL (P1: Leu-Lys-Phe-Leu), QLLF (P2: Gln-Leu-Leu-Phe), WLAYG (P3: Trp-Leu-Ala-Tyr-Gly), and HFCGG (P4: His-Phe-Cys-Gly-Gly). The vitro analysis and molecular docking revealed that peptides P1-P4 possessed remarkable scavenging ability against radicals and Fe 2+ chelating ability. Notably, peptide P4 showed radical scavenging activity comparable to glutathione (GSH) against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS) radicals. Cellular experiments further confirmed that peptide P4 effectively protected HepG2 cells from oxidative stress-induced damage. The modified rice husk biochar proved to be an effective means for enriching rice antioxidant peptides from protein hydrolysates.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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