| Autor: |
Dahal S; University of Nebraska-Lincoln, Department of Plant Pathology, Lincoln, Nebraska, United States.; University of Nebraska-Lincoln, Center for Plant Science Innovation, Lincoln, Nebraska, United States; sdahal4@huskers.unl.edu., Alvarez S; University of Nebraska-Lincoln, Center for Biotechnology, Lincoln, Nebraska, United States; salvarez@unl.edu., Balboa SJ; The University of North Carolina at Chapel Hill, Department of Chemistry, Chapel Hill, North Carolina, United States; sjbalboa@live.unc.edu., Hicks LM; The University of North Carolina at Chapel Hill, Chemistry, Chapel Hill, North Carolina, United States; lmhicks@unc.edu., Rojas CM; University of Nebraska-Lincoln, Plant Pathology, Lincoln, Nebraska, United States.; University of Nebraska-Lincoln, Center for Plant Science Innovation, Lincoln, Nebraska, United States; crojas2@unl.edu. |
| Abstrakt: |
Rice production worldwide is threatened by the disease Bacterial Panicle Blight (BPB) caused by Burkholderia glumae . Despite the threat, resources to control this disease such as completely resistant cultivars or effective chemical methods are still lacking. However, the need to control this disease has paved the way to explore biologically based approaches harnessing the antimicrobial activities of environmental bacteria. Previously, the bacterium Pseudomonas protegens PBL3 was identified as a potential biological control agent against B. glumae due to its antimicrobial activity against B. glumae . Such antimicrobial activity in vitro and in planta was associated with the P. protegens PBL3 bacteria-free secreted fraction (secretome), although the specific molecules responsible for this activity have remained elusive. In this work, we advance the characterization of the P. protegens PBL3 secretome, by evaluating the antimicrobial activity in vitro of selected secondary metabolites predicted by the P. protegens PBL3 genomic sequence against B. glumae . In addition, using Reversed Phase Liquid Chromatography Tandem Mass Spectrometry (RPLC-MS/MS), of the P. protegens PBL3 secretome, enabled us to successfully detect and quantify Pyoluteorin, 2,4-diacetylphloroglucinol (2,4-DAPG) and Pyochelin. Among those, Pyoluteorin and 2,4-DAPG reduced the growth of B. glumae in vitro along with reducing the symptoms of BPB and bacterial growth in planta, suggesting that these compounds could be effective as biopesticides to mitigate BPB. |
