Characterization of a Bifidobacterium animalis subsp. lactis reference strain based on ecology and transcriptomics.

Autor:	Lugli GA; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.; Microbiome Research Hub, University of Parma, Parma, Italy., Argentini C; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy., Tarracchini C; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy., Mancabelli L; Microbiome Research Hub, University of Parma, Parma, Italy.; Department of Medicine and Surgery, University of Parma, Parma, Italy., Viappiani A; GenProbio srl, Parma, Italy., Anzalone R; GenProbio srl, Parma, Italy., Angelini L; GenProbio srl, Parma, Italy., Alessandri G; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy., Longhi G; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy., Bianchi MG; Microbiome Research Hub, University of Parma, Parma, Italy.; Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy., Taurino G; Microbiome Research Hub, University of Parma, Parma, Italy.; Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy., Bussolati O; Microbiome Research Hub, University of Parma, Parma, Italy.; Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy., Milani C; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.; Microbiome Research Hub, University of Parma, Parma, Italy., Turroni F; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.; Microbiome Research Hub, University of Parma, Parma, Italy., Ventura M; Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.; Microbiome Research Hub, University of Parma, Parma, Italy.
Jazyk:	angličtina
Zdroj:	Applied and environmental microbiology [Appl Environ Microbiol] 2024 Oct 23; Vol. 90 (10), pp. e0108024. Date of Electronic Publication: 2024 Sep 05.
DOI:	10.1128/aem.01080-24
Abstrakt:	Bifidobacteria are recognized as health-promoting bacteria that reside in the human gut, helping in the digestion of fiber, preventing infections, and producing essential compounds like vitamins. To date, Bifidobacterium animalis subsp. lactis , together with Bifidobacterium adolescentis , Bifidobacterium bifidum , Bifidobacterium breve, and Bifidobacterium longum , represents one of the species that are used as probiotic bacteria. Despite the extensive and detailed scientific research conducted on this microbial taxon, the molecular mechanisms by which B. animalis subsp. lactis exerts health benefits to its host are still largely unknown. Thus, we dissected the genetic repertoire and phylogenetic relationship of 162 strains of B. animalis subsp. lactis to select a representative reference strain of this taxon suitable for investigating its interaction with the host. The B. animalis subsp. lactis PRL2013 strain, which was isolated by a mucosal sample of a healthy adult, was chosen as the reference of the monophyletic cluster of human origin and revealed a greater adhesion index than that observed for another B. animalis subsp. lactis strain used in the industry as a probiotic supplement. Transcriptomics analyses of PRL2013 strain, when exposed to human cell monolayers, revealed 291 significantly upregulated genes, among which were found genes predicted to encode extracellular structures that may directly interact with human cells, such as extracellular polymeric substances, wall teichoic acids, and pili. Importance: To date, many Bifidobacterium animalis subsp. lactis strains have been isolated from human fecal samples. However, their presence in these samples does not necessarily suggest an ability to colonize the human gut. Furthermore, probiotics of non-human origin may not effectively interact with the gut epithelium, resulting in transient bacteria of the gut microbiota. In vitro experiments with human cells revealed that B. animalis subsp. lactis PRL2013, an autochthonous member of the human gut, shows colonization capability, leading to future applications in functional foods. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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