| Autor: |
Melo FD; Centro de Diagnóstico Microbiológico Animal (CEDIMA), Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, Lages, Brazil., Peterle H; Centro de Diagnóstico Microbiológico Animal (CEDIMA), Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, Lages, Brazil., Sfaciotte RAP; Centro de Diagnóstico Microbiológico Animal (CEDIMA), Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, Lages, Brazil., Schneider MF; Centro de Diagnóstico Microbiológico Animal (CEDIMA), Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, Lages, Brazil., Parussolo L; Instituto Federal de Santa Catarina (IFSC), Florianópolis, Brazil., Wosiacki SR; Universidade Estadual de Maringa (UEM), São Cristovão, Brazil., Costa UMD; Centro de Diagnóstico Microbiológico Animal (CEDIMA), Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, Lages, Brazil., Ferraz SM; Centro de Diagnóstico Microbiológico Animal (CEDIMA), Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, Lages, Brazil. |
| Abstrakt: |
Staphylococcus aureus and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in Staphylococcus coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates ( n = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes ( mec A, mec C, and bla Z) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for mec A, whereas 10 strains (4 CoPS and 6 CoNS) were positive for bla Z. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines. |
