Cell-Permeable Nicotinamide Adenine Dinucleotides for Exploration of Cellular Protein ADP-Ribosylation.

Autor: Kasprzyk R; Department of Chemistry, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany., Rieth S; Department of Chemistry, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany.; Konstanz Research School Chemical Biology, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany., Heid P; Konstanz Research School Chemical Biology, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany.; Department of Biology, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany., Stengel F; Konstanz Research School Chemical Biology, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany.; Department of Biology, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany., Marx A; Department of Chemistry, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany.; Konstanz Research School Chemical Biology, University of Konstanz, Universitätstraβe 10, 78464, Konstanz, Germany.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2024 Dec 16; Vol. 63 (51), pp. e202411203. Date of Electronic Publication: 2024 Oct 25.
DOI: 10.1002/anie.202411203
Abstrakt: Posttranslational modifications (PTMs) greatly enhance the functional diversity of proteins, surpassing the number of gene-encoded variations. One intriguing PTM is ADP-ribosylation, which utilizes nicotinamide adenine dinucleotide (NAD + ) as a substrate and is essential in cell signaling pathways regulating cellular responses. Here, we report the first cell-permeable NAD + analogs and demonstrate their utility for investigating cellular ADP-ribosylation. Using a desthiobiotin-labelled analog for affinity enrichment of proteins that are ADP-ribosylated in living cells under oxidative stress, we identified protein targets associated with host-virus interactions, DNA damage and repair, protein biosynthesis, and ribosome biogenesis. Most of these targets have been noted in various literature sources, highlighting the potential of our probes for cellular ADP-ribosylome studies.
(© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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