Understanding the amylose biosynthesis and regulation mechanisms in Tartary buckwheat by the endosperm transcriptome.

Autor: Wang L; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Mao Y; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Zhou S; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Liu L; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Wang T; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Li C; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Wu H; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Zhao H; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China., Wang A; Xichang University, 615013 Xichang, Sichuan, China., Li S; Xichang University, 615013 Xichang, Sichuan, China., Wu Q; College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China. Electronic address: wuqi@sicau.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 279 (Pt 2), pp. 135275. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1016/j.ijbiomac.2024.135275
Abstrakt: Starch serves as a crucial energy source for both plants and humans, predominantly synthesized and stored in endosperms, tubers, rhizomes, and cotyledons. Given the significant role of amylose in determining the quality of starchy crops, optimizing its content has become a key objective in current crop breeding efforts. Tartary buckwheat, a dicotyledonous plant, notably accumulates high levels of amylose in its endosperm, surpassing common cereals like rice and maize. However, the mechanisms underlying amylose accumulation, distribution, and regulation in Tartary buckwheat remain unclear. Here, amylose content was determined across various tissues and organs of Tartary buckwheat, identifying with the endosperm as the primary site for its biosynthesis and accumulation. RNA sequencing analysis of endosperms from different developmental stages identified 35 genes potentially involved in starch biosynthesis, with 13 genes showing high endosperm-specific expression, suggesting crucial roles in starch biosynthesis. Additionally, the transcription factor FtNF-YB2, which was specifically highly expressed in the endosperm, was discovered to enhance amylose synthesis. Moreover, promoters with potential endosperm-specific activity were identified, advancing our understanding of amylose regulation. Additionally, this study also demonstrates that brassinosteroids (BR) positively influence amylose biosynthesis in Tartary buckwheat endosperm. These findings provide essential insights into the mechanisms of understanding amylose biosynthesis, accumulation and regulation in Tartary buckwheat, offering significant implications for future breeding strategies.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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