Enhanced enzymatic electrochemical detection of an organophosphate Pesticide: Achieving Wide linearity and femtomolar detection via gold nanoparticles growth within polypyrrole films.

Autor: Loguercio LF; Laboratório de Materiais Aplicados e Interfaces, Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, CP 15003, Porto Alegre, RS, Brazil., Griep J; Laboratório de Materiais Aplicados e Interfaces, Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, CP 15003, Porto Alegre, RS, Brazil., Demingos PG; Laboratório de Materiais Aplicados e Interfaces, Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, CP 15003, Porto Alegre, RS, Brazil., Morawski R; Laboratório de Materiais Aplicados e Interfaces, Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, CP 15003, Porto Alegre, RS, Brazil., Brolo AG; Department of Chemistry and Center for Advanced Materials and Related Technologies, University of Victoria, P.O. Box 3065, Victoria, V8W 3V6, BC, Canada., Andrade GFS; Laboratório de Nanoestruturas Plasmônicas, Núcleo de Espectroscopia e Estrutura Molecular, Dept. de Química, Universidade Federal de Juiz de Fora, CEP 36036-900, Juiz De Fora, MG, Brazil., Santos JFL; Laboratório de Materiais Aplicados e Interfaces, Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, CP 15003, Porto Alegre, RS, Brazil. Electronic address: jacqueline.ferreira@ufrgs.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2025 Jan 01; Vol. 281, pp. 126714. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1016/j.talanta.2024.126714
Abstrakt: The indiscriminate use of pesticides in agriculture demands the development of devices capable of monitoring contaminations in food supplies, in the environment and biological fluids. Simplicity, easy handling, high sensitivities, and low limits-of-detection (LOD) and quantification are some of the required properties for these devices. In this work, we evaluated the effect of incorporating gold nanoparticles into indigo carmine-doped polypyrrole during the electropolymerization of films for use as an acetylcholinesterase (AChE) enzyme-based biosensor. As proof of concept, the pesticide methyl parathion was tested towards the inhibition of AChE. The enzyme was immobilized simply by drop-casting a solution, eliminating the need for any prior surface modification. The biosensors were characterized with cyclic voltammetry, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The assays for the detection of methyl parathion with films containing polypyrrole, indigo carmine and AChE (PPy-IC-AChE) presented a sensitivity of 5.7 μA cm -2 g -1 mL and a LOD of 12 nmol L -1 (3.0 ng L -1 ) with a linear range from 1.3 x 10 -7  mol L -1 to 1.0 x 10 -5  mol L -1 . The introduction of gold nanoparticles (AuNP) into the film (PPy-IC-AuNP-AChE) led to remarkable improvements on the overall performance, such as a lower redox potential for the enzymatic reaction, a 145 % increase in sensitivity (14 μA cm -2  g -1 mL), a wider detection dynamic range (from 1.3x10 -7 to 1.0x10 -3  mol L -1 ), and a very low LOD of 24 fmol L -1 (64 ag mL -1 ). These findings underscore the potential of using AuNPs to improve the enzymatic performance of biosensor devices.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: