| Autor: |
Prosdocimi E; Department of Biology, University of Padova, Padova, Italy., Carpanese V; Department of Biology, University of Padova, Padova, Italy., Todesca LM; Department of Biology, University of Padova, Padova, Italy., Varanita T; Department of Biology, University of Padova, Padova, Italy., Bachmann M; Department of Biology, University of Padova, Padova, Italy., Festa M; Department of Biology, University of Padova, Padova, Italy., Bonesso D; Department of Biology, University of Padova, Padova, Italy., Perez-Verdaguer M; Department of Biology, University of Padova, Padova, Italy., Carrer A; Department of Biology, University of Padova, Padova, Italy.; Department of Biomedical Sciences, University of Padova, Padova, Italy., Velle A; Department of Biology, University of Padova, Padova, Italy., Peruzzo R; Department of Biology, University of Padova, Padova, Italy., Muccioli S; Department of Biology, University of Padova, Padova, Italy., Doni D; Department of Biology, University of Padova, Padova, Italy., Leanza L; Department of Biology, University of Padova, Padova, Italy., Costantini P; Department of Biology, University of Padova, Padova, Italy., Stein F; EMBL, Heidelberg, Germany., Rettel M; EMBL, Heidelberg, Germany., Felipe A; Molecular Physiology Laboratory, Department de Bioquímica i Biomedicina Molecular, Institut de Biomedicina (IBUB), Universitat de Barcelona, Barcelona, Spain., Edwards MJ; Department of Surgery, University of Cincinnati, Cincinnati, OH, USA., Gulbins E; Department of Molecular Biology, University of Duisburg-Essen, Essen, Germany., Cendron L; Department of Biology, University of Padova, Padova, Italy., Romualdi C; Department of Biology, University of Padova, Padova, Italy., Checchetto V; Department of Biology, University of Padova, Padova, Italy., Szabo I; Department of Biology, University of Padova, Padova, Italy. |
| Abstrakt: |
Kv1.3 is a multifunctional potassium channel implicated in multiple pathologies, including cancer. However, how it is involved in disease progression is not fully clear. We interrogated the interactome of Kv1.3 in intact cells using BioID proximity labeling, revealing that Kv1.3 interacts with STAT3- and p53-linked pathways. To prove the relevance of Kv1.3 and of its interactome in the context of tumorigenesis, we generated stable melanoma clones, in which ablation of Kv1.3 remodeled gene expression, reduced proliferation and colony formation, yielded fourfold smaller tumors, and decreased metastasis in vivo in comparison to WT cells. Kv1.3 deletion or pharmacological inhibition of mitochondrial Kv1.3 increased mitochondrial Reactive Oxygen Species release, decreased STAT3 phosphorylation, stabilized the p53 tumor suppressor, promoted metabolic switch, and altered the expression of several BioID-identified Kv1.3-networking proteins in tumor tissues. Collectively, our work revealed the tumor-promoting Kv1.3-interactome landscape, thus opening the way to target Kv1.3 not only as an ion-conducting entity but also as a signaling hub. |
