Identification and characterization of sulphotransferase (SOT) genes for tolerance against drought and heat in wheat and six related species.

Autor: Chaudhary J; Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250004, India., Gangwar H; Council of Scientific & Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, India., Jaiswal V; Council of Scientific & Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, India., Gupta PK; Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250004, India. pkgupta36@gmail.com.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Sep 04; Vol. 51 (1), pp. 956. Date of Electronic Publication: 2024 Sep 04.
DOI: 10.1007/s11033-024-09899-3
Abstrakt: Background: Sulphotransferase (SOT) enzyme (encoded by a conserved family of SOT genes) is involved in sulphonation of a variety of compounds, through transfer of a sulphuryl moiety from 3'phosphoadenosine- 5'phosphosulphate (PAPS) to a variety of secondary metabolites. The PAPS itself is derived from 3'adenosine-5'phosphosulphate (APS) that is formed after uptake of sulphate ions from the soil. The process provides tolerance against abiotic stresses like drought and heat in plants. Therefore, a knowledge of SOT genes in any crop may help in designing molecular breeding methods for improvement of tolerance for drought and heat.
Methods: Sequences of rice SOT genes and SOT domain (PF00685) of corresponding proteins were both used for identification of SOT genes in wheat and six related species (T. urartu, Ae. tauschii, T. turgidum, Z. mays, B. distachyon and Hordeum vulgare), although detailed analysis was conducted only in wheat. The wheat genes were mapped on individual chromosomes and also subjected to synteny and collinearity analysis. The proteins encoded by these genes were examined for the presence of a complete SOT domain using 'Conserved Domain Database' (CDD) search tool at NCBI.
Results: In wheat, 107 TaSOT genes, ranging in length from 969 bp to 7636 bp, were identified and mapped onto individual chromosomes. SSRs (simple sequence repeats), microRNAs, long non-coding RNAs (lncRNAs) and their target sites were also identified in wheat SOT genes. SOT proteins were also studied in detail. An expression assay of TaSOT genes via wheat RNA-seq data suggested engagement of these genes in growth, development and responses to various hormones and biotic/abiotic stresses.
Conclusions: The results of the present study should help in further functional characterization of SOT genes in wheat and other related crops.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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