Toward Analysis at the Point of Need: A Digital Microfluidic Approach to Processing Multi-Source Sexual Assault Samples.

Autor: Elsayed M; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3E2, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON, M5S 3E1, Canada.; Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON, M5S 3H6, Canada., Bodo L; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON, M5S 3E1, Canada.; Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON, M5S 3H6, Canada., Gaoiran C; Forensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada., Keuhnelian P; Forensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada., Dosajh A; Forensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada., Luk V; Forensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada., Schwandt M; ANDE Corporation, 1860 Industrial Circle, Suite A, Longmont, CO, 80501, USA., French JL; ANDE Corporation, 1860 Industrial Circle, Suite A, Longmont, CO, 80501, USA., Ghosh A; Centre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada., Erickson B; Centre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada., Charlesworth AG; Centre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada., Millman J; Centre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada., Wheeler AR; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3E2, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON, M5S 3E1, Canada.; Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON, M5S 3H6, Canada.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Nov; Vol. 11 (41), pp. e2405712. Date of Electronic Publication: 2024 Sep 04.
DOI: 10.1002/advs.202405712
Abstrakt: Forensic case samples collected in sexual assaults typically contain DNA from multiple sources, which complicates short-tandem repeat (STR) profiling. These samples are typically sent to a laboratory to separate the DNA from sperm and non-sperm sources prior to analysis. Here, the automation and miniaturization of these steps using digital microfluidics (DMF) is reported, which may eventually enable processing sexual assault samples outside of the laboratory, at the point of need. When applied to vaginal swab samples collected up to 12 h post-coitus (PC), the new method identifies single-source (male) STR profiles. When applied to samples collected 24-72 h PC, the method identifies mixed STR profiles, suggesting room for improvement and/or potential for data deconvolution. In sum, an automated, miniaturized sample pre-processing method for separating the DNA contained in sexual assault samples is demonstrated. This type of automated processing using DMF, especially when combined with Rapid DNA Analysis, has the potential to be used for processing of sexual assault samples in hospitals, police offices, and other locations outside of the laboratory.
(© 2024 His Majesty the King in Right of Canada. ANDE Corporation and The Author(s). Advanced Science published by Wiley‐VCH GmbH. Reproduced with the permission of the Minister of Ontario Ministry of Solicitor General public safety division.)
Databáze: MEDLINE
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