The usefulness of anti-HCV signal to cut-off ratio in predicting hepatitis C viremia and the effect of genotype differences on signal to cut-off ratio.

Autor:	Sarıkaya B; University of Health Sciences, Sultan 2. Abdulhamid Han Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology - İstanbul, Turkey., Yatar İD; University of Health Sciences, Sultan 2. Abdulhamid Han Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology - İstanbul, Turkey., Yılmaz S; Gülhane Training and Research Hospital, Regional Blood Center - Ankara, Turkey., Tiryaki Y; University of Health Sciences, Sultan 2. Abdulhamid Han Training and Research Hospital, Department of Medical Microbiology - İstanbul, Turkey., Sarıkaya VA; Haydarpaşa Numune Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology - İstanbul, Turkey., Çetinkaya RA; Reyap Health Group, Department of Infectious Diseases and Clinical Microbiology - İstanbul, Turkey., Kırkık D; University of Health Sciences, Hamidiye Medicine Faculty, Department of Medical Biology - İstanbul, Turkey.
Jazyk:	angličtina
Zdroj:	Revista da Associacao Medica Brasileira (1992) [Rev Assoc Med Bras (1992)] 2024 Sep 02; Vol. 70 (8), pp. e20240370. Date of Electronic Publication: 2024 Sep 02 (Print Publication: 2024).
DOI:	10.1590/1806-9282.20240370
Abstrakt:	Objective: In the hepatitis C virus (HCV) diagnostic algorithm, an anti-HCV screening test is recommended first. In countries with low HCV prevalence, anti-HCV testing can often give false-positive results. This may lead to unnecessary retesting, increased costs, and psychological stress for patients. Methods: In this study, the most appropriate S/Co (signal-cutoff) value to predict HCV viremia in anti-HCV test(+) individuals was determined, and the effect of genotype differences was evaluated. Of the 96,515 anti-HCV tests performed between 2020 and 2023, 934 were reactive. A total of 332 retests and 65 patients without HCV-ribonucleic acid (RNA) analysis were excluded. Demographic data were calculated for 537 patients, and 130 patients were included in the study. Results: The average age of 537 patients was 55±18 years, and 57.1% were women. The anti-HCV positivity rate was 0.62% (602/96,515), and the actual anti-HCV positivity rate was 0.13% (130/96,515). Anti-HCV levels were higher in HCV-RNA(+) patients than in HCV-RNA-negative individuals (p<0.0001) (Table 1). Receiver operating characteristic curve analysis identified the optimal S/Co value to be 10.86 to identify true positive cases. Sensitivity was 96.1%, specificity was 61.2%, positive predictive value (PPV) was 44.2%, and negative predictive value (NPV) was 98% (Figure 2). A total of 107 (82.3%) of the patients were identified as GT1, and the most common subtype was GT1b (n=100). Conclusion: If anti-HCV S/Co is ≥10.86, direct HCV RNA testing may be recommended; However, the possibility of false positivity should be considered in patients with a S/Co value below 10.86.
Databáze:	MEDLINE
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