Keratinocyte-Derived Exosomes in Painful Diabetic Neuropathy.

Autor:	Coy-Dibley J; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Jayaraj ND; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Ren D; Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Pacifico P; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Belmadani A; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.; Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Wang YZ; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Gebis KK; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Savas JN; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Paller AS; Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Miller RJ; Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Menichella DM; Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.; Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 03. Date of Electronic Publication: 2024 Sep 03.
DOI:	10.1101/2024.08.21.608803
Abstrakt:	Painful diabetic neuropathy (PDN) is a challenging complication of diabetes with patients experiencing a painful and burning sensation in their extremities. Existing treatments provide limited relief without addressing the underlying mechanisms of the disease. PDN involves the gradual degeneration of nerve fibers in the skin. Keratinocytes, the most abundant epidermal cell type, are closely positioned to cutaneous nerve terminals, suggesting the possibility of bi-directional communication. Exosomes are small extracellular vesicles released from many cell types that mediate cell to cell communication. The role of keratinocyte-derived exosomes (KDEs) in influencing signaling between the skin and cutaneous nerve terminals and their contribution to the genesis of PDN has not been explored. In this study, we characterized KDEs in a well-established high-fat diet (HFD) mouse model of PDN using primary adult mouse keratinocyte cultures. We obtained highly enriched KDEs through size exclusion chromatography and then analyzed their molecular cargo using proteomic analysis and small RNA sequencing. We found significant differences in the protein and microRNA content of HFD KDEs compared to KDEs obtained from control mice on a regular diet (RD), including pathways involved in axon guidance and synaptic transmission. Additionally, using an in vivo conditional extracellular vesicle (EV) reporter mouse model, we demonstrated that epidermal-originating GFP-tagged KDEs are retrogradely trafficked into the DRG neuron cell body. Overall, our study presents a potential novel mode of communication between keratinocytes and DRG neurons in the skin, revealing a possible role for KDEs in contributing to the axonal degeneration that underlies neuropathic pain in PDN. Moreover, this study presents potential therapeutic targets in the skin for developing more effective, disease-modifying, and better-tolerated topical interventions for patients suffering from PDN, one of the most common and untreatable peripheral neuropathies. Competing Interests: Conflict of interest statement: The authors have declared that no conflict of interest exists.
Databáze:	MEDLINE
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