New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans .
| Autor: | Wittes J; Dept. of Biological Sciences, Columbia University, New York, New York, USA., Greenwald I; Dept. of Biological Sciences, Columbia University, New York, New York, USA. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2024 Aug 19; Vol. 2024. Date of Electronic Publication: 2024 Aug 19 (Print Publication: 2024). |
| DOI: | 10.17912/micropub.biology.001315 |
| Abstrakt: | A Flexon stop cassette interrupts translation of a coding region until it is excised by a recombinase to allow for gene expression. We have expanded options for Auxin-Inducible Degradation by generating Flexon-based transgenes for tissue-specific expression of the ubiquitin ligase substrate recognition component TIR1 or the variant TIR1(F79G) after excision of the Flexon by Cre recombinase. We also describe Flexon-based tester transgenes to facilitate gathering accurate information about the expression pattern of Cre and Flp recombinase drivers that can be used in conjunction with any conditional expression reagents that utilize these recombinases. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.) |
| Databáze: | MEDLINE |
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