Chitosan-enhanced sensitivity of mercaptoundecanoic acid (MUA)- capped gold nanorod based localized surface plasmon resonance (LSPR) biosensor for detection of alpha-synuclein oligomer biomarker in parkinson's disease.

Autor: Apaydın BB; Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey., Çamoğlu T; Department of Neuroscience, Institute of Neurological Sciences, Istanbul University-Cerrahpaşa, Istanbul, Turkey., Canbek Özdil ZC; Materials Science and Nanotechnology Engineering, Yeditepe University, Istanbul, Turkey., Gezen-Ak D; Department of Neuroscience, Institute of Neurological Sciences, Istanbul University-Cerrahpaşa, Istanbul, Turkey., Ege D; Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey., Gülsoy M; Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2024 Sep 03. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1002/bab.2653
Abstrakt: Alpha-synuclein oligomers play a crucial role in the early diagnosis of Parkinson's disease (PD). In this study, a mercaptoundecanoic acid (MUA)-capped gold nanorod (GNR)-coated and chitosan (CH)-immobilized fiber optic probe has shown considerable sensitivity of its detection. The proposed U-shaped fiber optic biosensor based on localized surface plasmon resonance (LSPR) was applied to detect α-syn oligomer (OA) biomarker. By analyzing OA concentrations, the biosensor achieved a limit of detection of (LOD) 11 pM within the concentration range of 10-100 pM and the sensitivity value was found as 502.69 Δλ/RIU. Upon analysis of the CV% (coefficient of variation) and accuracy/recovery values, it is revealed that the sensor successfully fulfilled the criteria for success, displaying accuracy/recovery values within the range of 80%-120% and CV% values below 20%. This sensor presents significant advantages, including high sensitivity, specificity, and ability to detect very low concentrations of OA. In conclusion, the suggested U-shaped fiber optic biosensor has the potential to be valuable in the early detection of PD from a clinical perspective.
(© 2024 The Author(s). Biotechnology and Applied Biochemistry published by Wiley Periodicals LLC on behalf of International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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