| Autor: |
Kaestli M; Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia., Farook S; Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh., Jilani MSA; Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh., Anwar S; Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh., Siddiqui TA; Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh., Mayo M; Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia., Podin Y; Institute of Health and Community Medicine, Universiti Malaysia Sarawak, Sarawak, Malaysia., Webb JR; Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.; Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Dance DAB; Lao-Oxford-Mahosot Hospital-Wellcome Trust Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom., Currie BJ; Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.; Infectious Diseases Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia. |
| Abstrakt: |
Melioidosis is an emerging tropical infectious disease with a rising global burden caused by the environmental bacterium Burkholderia pseudomallei. It is endemic in Southeast and South Asia, including Bangladesh. A rare aminoglycoside-susceptible B. pseudomallei isolate (Y2019) has recently been reported from a melioidosis patient in Dhaka, Bangladesh. To understand the geographical origins of Y2019, we subjected it and 10 other isolates from Bangladesh to whole-genome sequencing. In a phylogenetic tree with a global set of B. pseudomallei genomes, most Bangladeshi genomes clustered tightly within the Asian clade. In contrast, Y2019 was closely related to ST881 isolates from Sarawak, Malaysian Borneo, a gentamicin-sensitive sequence type, suggesting infection in Borneo. Y2019 also contained the same gentamicin sensitivity conferring nonsynonymous mutation in the drug efflux pump encoding the amrB gene. In the absence of a full travel history, whole-genome sequencing and bioinformatics tools have revealed the likely origin of this rare isolate. |
