| Autor: |
Choudhury S; Department of Bioengineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India., Joshi A; Department of Bioengineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India., Agrawal A; Department of Bioengineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India., Nain A; Department of Materials Engineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India., Bagde A; Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research, Wardha 442001, India., Patel A; Department of Conservative Dentistry and Endodontics, Sharad Pawar Dental College, Datta Meghe Institute of Higher Education and Research, Wardha 442001, India., Syed ZQ; Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research, Wardha 442001, India.; Department of Community Medicine, Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research, Wardha 442001, India., Asthana S; Department of Materials Engineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India.; Department of Hepatobiliary and Multi-Organ Transplantation Surgery, Aster CMI Hospital, Bangalore 560024, India., Chatterjee K; Department of Bioengineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India.; Department of Materials Engineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India.; Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research, Wardha 442001, India. |
| Abstrakt: |
The treatment of irregular-shaped and critical-sized bone defects poses a clinical challenge. Deployable, self-fitting tissue scaffolds that can be implanted by minimally invasive procedures are a promising solution. Toward this, we fabricated NIR-responsive and programmable polylactide-co-trimethylene carbonate (PLMC) scaffolds nanoengineered with polydopamine nanoparticles (PDA) by extrusion-based three-dimensional (3D) printing. The 3D-printed scaffolds demonstrated excellent (>99%), fast (under 30 s), and tunable shape recovery under NIR irradiation. PLMC-PDA composites demonstrated significantly higher osteogenic potential in vitro as revealed by the significantly enhanced alkaline phosphatase (ALP) secretion and mineral deposition in contrast to neat PLMC. Intraoperative deployability and in vivo bone regeneration ability of PLMC-PDA composites were demonstrated, using self-fitting scaffolds in critical-sized cranial bone defects in rabbits. The 3D-printed scaffolds were deformed into compact shapes that could self-fit into the defect shape intraoperatively under low power intensity (0.76 W cm -2 ) NIR. At 6 and 12 weeks postsurgical implantation, near-complete bone regeneration was observed in PLMC-PDA composites, unlike neat PLMC through microcomputed tomography (micro-CT) analysis. The potential clinical utility of the 3D-printed composites to secure complex defects was confirmed through self-fitting of the scaffolds into irregular defects in ex vivo models of rabbit tibia, mandible, and tooth models. Taken together, the composite scaffolds fabricated here offer an innovative strategy for minimally invasive deployment to fit irregular and complex tissue defects for bone tissue regeneration. |
