Chick Embryo Chorioallantoic Membrane as a Platform for Assessing the In Vivo Efficacy of Chimeric Antigen Receptor T-cell Therapy in Solid Tumors.

Autor:	Nipper AJ; Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston, TX., Warren EAK; Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, TX., Liao KS; Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, TX., Liu HC; Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, TX., Michikawa C; Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston, TX., Porter CE; Department of Family and Community Medicine, Baylor College of Medicine, Houston, TX.; Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX., Wells GA; Advanced Technology Cores, Baylor College of Medicine, Houston, TX., Villanueva M; Department of Family and Community Medicine, Baylor College of Medicine, Houston, TX., Brasil da Costa FH; Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston, TX., Veeramachaneni R; Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston, TX., Villanueva H; Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, TX.; Advanced Technology Cores, Baylor College of Medicine, Houston, TX., Suzuki M; Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX.; Texas Children's Hospital, Houston, TX.; Dan L. Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX., Sikora AG; Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston, TX.
Jazyk:	angličtina
Zdroj:	ImmunoHorizons [Immunohorizons] 2024 Aug 01; Vol. 8 (8), pp. 598-605.
DOI:	10.4049/immunohorizons.2400059
Abstrakt:	The fertilized chicken egg chorioallantoic membrane (CAM), a highly vascularized membrane nourishing the developing embryo, also supports rapid growth of three-dimensional vascularized tumors from engrafted cells and tumor explants. Because murine xenograft models suffer limitations of time, cost, and scalability, we propose CAM tumors as a rapid, efficient screening tool for assessing anti-tumor efficacy of chimeric Ag receptor (CAR) T cells against solid tumors. We tested the efficacy of human epidermal growth factor receptor 2 (HER2)-specific CAR T cells against luminescent, HER2-expressing (FaDu, SCC-47) or HER2-negative (MDA-MB-468) CAM-engrafted tumors. Three days after tumor engraftment, HER2-specific CAR T cells were applied to tumors grown on the CAM. Four days post-CAR T cell treatment, HER2-expressing FaDu and SCC-47 tumors treated with CAR T showed reduced viable cancer cells as assessed by luciferase activity. This reduction in viable tumor cells was confirmed by histology, with lower Ki-67 staining observed in CAR T cell-treated tumors relative to T cell-treated controls. Persistence of CAR T in CAM and tumor tissue 4 days post-treatment was confirmed by CD3 staining. Altogether, our findings support further development of the chick CAM as an in vivo system for rapid, scalable screening of CAR T cell efficacy against human solid tumors. (Copyright © 2024 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu