Autofluorescent Cancer Stem Cells: Potential Biomarker to Predict Recurrence in Resected Colorectal Tumors.

Autor:	Alcala S; Department of Biochemistry, School of Medicine, Autónoma University of Madrid and Department of Cancer, Instituto de Investigaciones Biomédicas (IIBm) Sols-Morreale (CSIC-UAM), Madrid, Spain.; Biomarkers and Personalized Approach to Cancer (BIOPAC) Group, Area 3 Cancer, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain., Serralta San Martin G; Department of Internal Medicine, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain.; Universidad Europea de Madrid, Madrid, Spain., Muñoz-Fernández de Legaria M; Department of Pathology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain., Moreno-Rubio J; Department of Medical Oncology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain.; Precision Nutrition and Cancer Program, Clinical Oncology Group, IMDEA Food Institute, CEI UAM-CSIC, Madrid, Spain., Salinas S; Department of Pathology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain., López-Gil JC; Department of Biochemistry, School of Medicine, Autónoma University of Madrid and Department of Cancer, Instituto de Investigaciones Biomédicas (IIBm) Sols-Morreale (CSIC-UAM), Madrid, Spain.; Biomarkers and Personalized Approach to Cancer (BIOPAC) Group, Area 3 Cancer, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain., Rojo López JA; Department of General Surgery, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain., Martínez Alegre J; Universidad Europea de Madrid, Madrid, Spain.; Department of General Surgery, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain., Cortes Bandy DA; Department of Medical Oncology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain., Zambrana F; Universidad Europea de Madrid, Madrid, Spain.; Department of Medical Oncology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain.; Precision Nutrition and Cancer Program, Clinical Oncology Group, IMDEA Food Institute, CEI UAM-CSIC, Madrid, Spain., Jiménez-Gordo AM; Universidad Europea de Madrid, Madrid, Spain.; Department of Medical Oncology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain.; Precision Nutrition and Cancer Program, Clinical Oncology Group, IMDEA Food Institute, CEI UAM-CSIC, Madrid, Spain., Casado E; Universidad Europea de Madrid, Madrid, Spain.; Department of Medical Oncology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain.; Precision Nutrition and Cancer Program, Clinical Oncology Group, IMDEA Food Institute, CEI UAM-CSIC, Madrid, Spain., López-Gómez M; Universidad Europea de Madrid, Madrid, Spain.; Department of Medical Oncology, Infanta Sofía University Hospital, FIIB HUIS HHEN, Madrid, Spain., Sainz B; Department of Biochemistry, School of Medicine, Autónoma University of Madrid and Department of Cancer, Instituto de Investigaciones Biomédicas (IIBm) Sols-Morreale (CSIC-UAM), Madrid, Spain.; Biomarkers and Personalized Approach to Cancer (BIOPAC) Group, Area 3 Cancer, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; Centro de Investigación Biomédica en Red, Área Cáncer, CIBERONC, ISCIII, Madrid, Spain.
Jazyk:	angličtina
Zdroj:	Cancer research communications [Cancer Res Commun] 2024 Oct 01; Vol. 4 (10), pp. 2575-2588.
DOI:	10.1158/2767-9764.CRC-24-0188
Abstrakt:	Cancer stem cells (CSC) in colorectal cancer drive intratumoral heterogeneity and distant metastases. Previous research from our group showed that CSCs can be easily detected by autofluorescence (AF). The aim of the present study was to evaluate the potential role of AF CSCs as a prognostic biomarker for colorectal cancer relapse. Seventy-five freshly resected tumors were analyzed by flow cytometry. AF was categorized as high (H-AF) or low, and the results were correlated with histologic features [grade of differentiation, presence of metastases in lymph nodes (LN), perivascular and lymphovascular invasion] and clinical variables (time to relapse and overall survival). Nineteen of the 75 (25.3%) patients experienced relapse (local or distant); of these 19 patients, 13 showed positive LNs and 6 had H-AF. Of note, four of them died before 5 years. Although patients with H-AF CSC percentages in the global population experienced 1.5 times increased relapse [HR, 1.47; 95% confidence interval (0.60-3.63)], patients with H-AF CSC percentages and LN metastases had the highest risk of relapse [HR, 7.92; P < 0.004; 95% confidence interval (1.97-31.82)]. These data support AF as an accurate and feasible marker to identify CSCs in resected colorectal cancer. A strong statistical association between H-AF CSCs and the risk of relapse was observed, particularly in patients with positive LNs, suggesting that H-AF patients might benefit from adjuvant chemotherapy regimens and intensive surveillance due to their high propensity to experience disease recurrence. Significance: AF has been proven to be an accurate biomarker for CSC identification; however, to date, their role as a prognostic factor after resection of colorectal cancer tumors has not been investigated. Our results show that determining the presence of AF CSCs after tumor resection has prognostic value and represents a potentially important tool for the management of patients with colorectal cancer. (©2024 The Authors; Published by the American Association for Cancer Research.)
Databáze:	MEDLINE
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