Medicago truncatula SOBIR1 controls pathogen immunity and specificity in the Rhizobium-legume symbiosis.

Autor: Sarrette B; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Luu TB; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Johansson A; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Fliegmann J; Centre for Plant Molecular Biology (ZMBP) - Plant Biochemistry, University of Tübingen, Tübingen, Germany., Pouzet C; Fédération de Recherche Agrobiosciences, Interactions and Biodiversity Research (FR AIB) Imaging and Proteomics platforms, University of Toulouse III, CNRS, Auzeville-Tolosan, France., Pichereaux C; Fédération de Recherche Agrobiosciences, Interactions and Biodiversity Research (FR AIB) Imaging and Proteomics platforms, University of Toulouse III, CNRS, Auzeville-Tolosan, France.; Institut de Pharmacologie et de Biologie Structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Toulouse, France.; Infrastructure Nationale de Protéomique, ProFI, Toulouse, France., Remblière C; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Sauviac L; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Carles N; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Amblard E; Laboratoire de Recherche en Sciences Végétales, University of Toulouse, CNRS, UPS, Castanet-Tolosan, France., Guyot V; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Bonhomme M; Laboratoire de Recherche en Sciences Végétales, University of Toulouse, CNRS, UPS, Castanet-Tolosan, France., Cullimore J; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Gough C; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France., Jacquet C; Laboratoire de Recherche en Sciences Végétales, University of Toulouse, CNRS, UPS, Castanet-Tolosan, France., Pauly N; Laboratory of Plant-Microbe Interactions and Environment (LIPME), University Toulouse III, INRAE, CNRS, Castanet-Tolosan Cedex, France.; Institut Sophia Agrobiotech, Université Côte d'Azur, INRAE, CNRS, Sophia Antipolis Cedex, France.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Sep 03. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1111/pce.15071
Abstrakt: Medicago truncatula Nod Factor Perception (MtNFP) plays a role in both the Rhizobium-Legume (RL) symbiosis and plant immunity, and evidence suggests that the immune-related function of MtNFP is relevant for symbiosis. To better understand these roles of MtNFP, we sought to identify new interacting partners. We screened a yeast-2-hybrid cDNA library from Aphanomyces euteiches infected and noninfected M. truncatula roots. The M. truncatula leucine-rich repeat (LRR) receptor-like kinase SUPPRESSOR OF BIR1 (MtSOBIR1) was identified as an interactor of MtNFP and was characterised for kinase activity, and potential roles in symbiosis and plant immunity. We showed that the kinase domain of MtSOBIR1 is active and can transphosphorylate the pseudo-kinase domain of MtNFP. MtSOBIR1 could functionally complement Atsobir1 and Nbsobir1/sobir1-like mutants for defence activation, and Mtsobir1 mutants were defective in immune responses to A. euteiches. For symbiosis, we showed that Mtsobir1 mutant plants had both a strong, early infection defect and defects in the defence suppression in nodules, and both effects were plant genotype- and rhizobial strain-specific. This work highlights a conserved function for MtSOBIR1 in activating defence responses to pathogen attack, and potentially novel symbiotic functions of downregulating defence in association with the control of symbiotic specificity.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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