Monocyte derived large extracellular vesicles in polytrauma.

Autor: Wöhler A; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany., Gries SK; Department of Internal Medicine I University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany.; Department of Internal Medicine II Saarland University Medical Center, Saarland University Homburg Germany., Salzmann RJS; Department of Internal Medicine I University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany., Krötz C; Department of Internal Medicine II Saarland University Medical Center, Saarland University Homburg Germany., Wang B; Department of Internal Medicine I University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany., Müller P; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany., Klein A; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany., Schmidt-Wolf IGH; Department of Integrated Oncology, Center for Integrated Oncology (CIO) University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany., Schaaf S; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany., Schwab R; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany., Lukacs-Kornek V; Institute of Molecular Medicine and Experimental Immunology University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany., Willms AG; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany., Kornek MT; Department of General, Visceral and Thoracic Surgery German Armed Forces Central Hospital Koblenz Germany.; Department of Internal Medicine I University Hospital Bonn of the Rheinische Friedrich-Wilhelms-University Bonn Germany.; Department of Internal Medicine II Saarland University Medical Center, Saarland University Homburg Germany.
Jazyk: angličtina
Zdroj: Journal of extracellular biology [J Extracell Biol] 2024 Sep 02; Vol. 3 (9), pp. e70005. Date of Electronic Publication: 2024 Sep 02 (Print Publication: 2024).
DOI: 10.1002/jex2.70005
Abstrakt: Despite significant progress in the medical field, there is still a pressing need for minimal-invasive tools to assist with decision-making, especially in cases of polytrauma. Our team explored the potential of serum-derived large extracellular vesicles, so called microparticles/microvesicles/ectosomes, to serve as a supportive tool in decision-making in polytrauma situations. We focused on whether monocyte derived large EVs may differentiate between polytrauma patients with internal organ injury (ISS > 15) and those without. Thus, we compared our EV data to soluble biomarkers such as tumour necrosis factor alpha (TNF alpha) and Interleukin-8 (IL-8). From the blood of 25 healthy and 26 patients with polytrauma large EVs were isolated, purified, and characterized. TNF alpha and IL-8 levels were quantified. We found that levels of these monocyte derived large EVs were significantly higher in polytrauma patients with internal organ damage and correlated with the ISS. Interestingly, we also observed a decline in AnnV + CD14 + large EVs during normal recovery after trauma. Thus, inflammatory serological markers as TNF alpha and as IL-8 demonstrated an inability to discriminate between polytrauma patients with or without internal organ damage, such as spleen, kidney, or liver lacerations/ruptures. However, TNF and IL-8 levels were elevated in polytrauma cases overall when contrasted with healthy non-traumatic controls. These findings suggest that delving deeper into the potential of AnnV + large EVs derived from monocytes could highly beneficial in the managment of polytrauma, potentially surpassing the efficacy of commonly used serum markers.
Competing Interests: The authors declare no competing interests to disclose.
(© 2024 The Author(s). Journal of Extracellular Biology published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.)
Databáze: MEDLINE
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